TY - JOUR
T1 - Immunotoxic damage in floriculturists exposed to pesticide mixtures
AU - Castillo-Cadena, Julieta
AU - González-Mercado, Ana Laura
AU - Hernández-Caballero, Nancy
AU - Juan, Eduardo Ramírez San
AU - Álvarez-González, Isela
AU - Madrigal-Bujaidar, Eduardo
N1 - Funding Information:
The project was partially supported by UAEM, grant 2806/2009.
PY - 2013/1
Y1 - 2013/1
N2 - The aim of the present work was to determine if the use of pesticide mixtures produced alterations in immunotoxicity biomarkers. The study was undertaken in three groups: the first group consisted of 38 floriculturists, the second comprised 38 vendors of the local market, and the last comprised 33 non-exposed persons from another locality. The determinations included haematocrit, mean cellular volume, hemoglobin, number of erythrocytes and leukocytes, immunoglobulins (IgA, IgG, IgM, and IgE), percentage of T-lymphocytes, and mitotic index from lymphocyte cultures with and without phytohaemagglutinin (PHA). The obtained results indicated the following: (i) in the level of the studied immunoglobulins, the results were within the reference values; (ii) there was a reduction in the amount of T-lymphocytes in the floriculturists in comparison with the determined in the other two groups; (iii) there was a decrease in the mitotic index of PHA-stimulated lymphocyte cultures of floriculturists and vendors in relation to the value of the control group; (iv) there was an increase in the mitotic index of unstimulated lymphocytes of floriculturists compared with the lymphocytes of the other two groups; and (v) there was no correlation between the results and the personal characteristics of the studied individuals. Our results established an immunotoxic effect in the floriculturists exposed to pesticides.
AB - The aim of the present work was to determine if the use of pesticide mixtures produced alterations in immunotoxicity biomarkers. The study was undertaken in three groups: the first group consisted of 38 floriculturists, the second comprised 38 vendors of the local market, and the last comprised 33 non-exposed persons from another locality. The determinations included haematocrit, mean cellular volume, hemoglobin, number of erythrocytes and leukocytes, immunoglobulins (IgA, IgG, IgM, and IgE), percentage of T-lymphocytes, and mitotic index from lymphocyte cultures with and without phytohaemagglutinin (PHA). The obtained results indicated the following: (i) in the level of the studied immunoglobulins, the results were within the reference values; (ii) there was a reduction in the amount of T-lymphocytes in the floriculturists in comparison with the determined in the other two groups; (iii) there was a decrease in the mitotic index of PHA-stimulated lymphocyte cultures of floriculturists and vendors in relation to the value of the control group; (iv) there was an increase in the mitotic index of unstimulated lymphocytes of floriculturists compared with the lymphocytes of the other two groups; and (v) there was no correlation between the results and the personal characteristics of the studied individuals. Our results established an immunotoxic effect in the floriculturists exposed to pesticides.
KW - Pesticide mixtures
KW - T-lymphocytes
KW - floriculturists
KW - hematology profile
KW - immunoglobulins
KW - immunotoxicity
KW - proliferative responses
UR - http://www.scopus.com/inward/record.url?scp=84867205912&partnerID=8YFLogxK
U2 - 10.1080/03601234.2012.716690
DO - 10.1080/03601234.2012.716690
M3 - Artículo
C2 - 23030438
SN - 0360-1234
VL - 48
SP - 33
EP - 39
JO - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
JF - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
IS - 1
ER -