TY - GEN
T1 - Immobilization of SA-α-2,6-gal receptors related to influenza on magnetic nanoparticles coated with chitosan
AU - Gregorio-Jauregui, Karla M.
AU - Carrizalez-Alvarez, Susana A.
AU - Rivera-Salinas, Jorge E.
AU - Saade, Hened
AU - Martinez, José L.
AU - López, Raúl G.
AU - Ilyina, Anna
PY - 2014
Y1 - 2014
N2 - Influenza infection is carried out due to the virus hemagglutinin recognizes host cell surface with terminal sialic acid (SA) linked receptors SA-α-2,6-Gal for human strain. Sambucus nigra lectin has structural similarity to the viral hemagglutinin. Magnetic nanoparticles (MNP) coated with chitosan can be used as support for the study of these receptors. The goal of this study was to extract the SA-α-2,6-Gal receptors of porcine trachea for their immobilization on magnetic nanoparticles coated with chitosan. The extraction was carried out based on affinity of Sambucus nigra lectin to SA-α-2,6-Gal receptors. It was possible to immobilize up to 76% of SA-α-2,6-Gal receptors, with a molecular weight at 88.4 kDa for more representative protein. The presence of the receptors was confirmed trough FTIR analysis. Magnetic functionalized nanoparticles showed superparamagnetic properties and an average diameter around 10 nm. These results may be used to evaluate the interaction between functionalized nanoparticles and specific lectin or hemagglutinin of influenza virus as model of study virus-receptor.
AB - Influenza infection is carried out due to the virus hemagglutinin recognizes host cell surface with terminal sialic acid (SA) linked receptors SA-α-2,6-Gal for human strain. Sambucus nigra lectin has structural similarity to the viral hemagglutinin. Magnetic nanoparticles (MNP) coated with chitosan can be used as support for the study of these receptors. The goal of this study was to extract the SA-α-2,6-Gal receptors of porcine trachea for their immobilization on magnetic nanoparticles coated with chitosan. The extraction was carried out based on affinity of Sambucus nigra lectin to SA-α-2,6-Gal receptors. It was possible to immobilize up to 76% of SA-α-2,6-Gal receptors, with a molecular weight at 88.4 kDa for more representative protein. The presence of the receptors was confirmed trough FTIR analysis. Magnetic functionalized nanoparticles showed superparamagnetic properties and an average diameter around 10 nm. These results may be used to evaluate the interaction between functionalized nanoparticles and specific lectin or hemagglutinin of influenza virus as model of study virus-receptor.
KW - 6-gal receptors
KW - Magnetic nanoparticles
KW - SA-α-2
KW - Sambucus nigra lectin
UR - http://www.scopus.com/inward/record.url?scp=84904163079&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.976.19
DO - 10.4028/www.scientific.net/AMR.976.19
M3 - Contribución a la conferencia
AN - SCOPUS:84904163079
SN - 9783038351566
T3 - Advanced Materials Research
SP - 19
EP - 24
BT - Science and Materials Engineering IV
PB - Trans Tech Publications Ltd
T2 - 4th National Congress of Science and Materials Engineering, CNCIM 2013
Y2 - 22 February 2013 through 28 February 2013
ER -