TY - JOUR
T1 - Immobilization of cytochrome P-450 on MCM-41 with different silicon/aluminum ratios
AU - Hernández, M. C.Rosales
AU - Wejebe, J. E.Mendieta
AU - Alcántara, J. I.Vázquez
AU - Ruvalcaba, R. Miranda
AU - Serrano, L. A.García
AU - Ferrara, J. Trujillo
N1 - Funding Information:
The authors would like to thank IMP, COFAA and C.G.P.I for financial support, and also Dr. E. Terrés and Dr. C. Flores (Instituto Mexicano del Petróleo, IMP) for their assistance in the preparation of the manuscript.
PY - 2005
Y1 - 2005
N2 - The catalytic activity of two cytochrome P-450 (CYP450) isoforms (CYP2C9 and CYP2B4, 1.14.14.1) immobilized on a mesoporous material "MCM-41" synthesized with Si/Al ratios of ∞, 80 and 100 was evaluated. The amount of CYP2C9 and CYP2B4 immobilized in each material was different, due to isoform size, the number of hydrogen bonds and the electrostatic interactions between the aminoacid residues and the Lewis acid sites of the material. The catalytic activity of immobilized CYP2C9 and CYP2B4 took place without using cytochrome P-450 reductase; consequently MCM-41 participates in electron transfer through the Lewis acid sites. In addition, the catalytic activity of immobilized CYP2B4 on MCM-41 was similar to its free form when a Si/Al ratio of 100 was used. Finally, although the catalytic activity of immobilized CYP2C9 was also better on MCM-41 with a Si/Al ratio of 100, it maintained around 30% of the catalytic activity in relation to its free form.
AB - The catalytic activity of two cytochrome P-450 (CYP450) isoforms (CYP2C9 and CYP2B4, 1.14.14.1) immobilized on a mesoporous material "MCM-41" synthesized with Si/Al ratios of ∞, 80 and 100 was evaluated. The amount of CYP2C9 and CYP2B4 immobilized in each material was different, due to isoform size, the number of hydrogen bonds and the electrostatic interactions between the aminoacid residues and the Lewis acid sites of the material. The catalytic activity of immobilized CYP2C9 and CYP2B4 took place without using cytochrome P-450 reductase; consequently MCM-41 participates in electron transfer through the Lewis acid sites. In addition, the catalytic activity of immobilized CYP2B4 on MCM-41 was similar to its free form when a Si/Al ratio of 100 was used. Finally, although the catalytic activity of immobilized CYP2C9 was also better on MCM-41 with a Si/Al ratio of 100, it maintained around 30% of the catalytic activity in relation to its free form.
KW - Cytochrome P-450
KW - Electron transfer
KW - Enzymatic activity
KW - Enzyme immobilization
KW - MCM-41
UR - http://www.scopus.com/inward/record.url?scp=18844379930&partnerID=8YFLogxK
U2 - 10.1016/j.micromeso.2004.11.010
DO - 10.1016/j.micromeso.2004.11.010
M3 - Artículo
SN - 1387-1811
VL - 80
SP - 25
EP - 31
JO - Microporous and Mesoporous Materials
JF - Microporous and Mesoporous Materials
IS - 1-3
ER -