TY - JOUR
T1 - Identification of the Na+/H+ exchanger 1 in dorsal root ganglion and spinal cord
T2 - Its possible role in inflammatory nociception
AU - Rocha-González, H. I.
AU - Castañeda-Corral, G.
AU - Araiza-Saldaña, C. I.
AU - Ambriz-Tututi, M.
AU - Caram-Salas, N. L.
AU - Torres-López, J. E.
AU - Murbartián, J.
AU - Granados-Soto, V.
N1 - Funding Information:
The authors greatly appreciate the bibliographic and technical assistance of Héctor Vázquez and Guadalupe C. Vidal-Cantú, respectively. Héctor I. Rocha-González, Claudia I. Araiza-Saldaña, Nadia L. Caram-Salas, Mónica Ambriz-Tututi and Gabriela Castañeda-Corral are Conacyt fellows. Claudia I. Araiza-Saldaña and Nadia L. Caram-Salas are recipients of the “Apoyos Integrales para la Formación de Doctores en Ciencias” fellowship. Partially supported by Conacyt grants 59879 (V.G.-S.) and 49084 (J.M.).
PY - 2009/4/21
Y1 - 2009/4/21
N2 - mRNA and protein presence of Na+/H+ exchanger (NHE) 1 (NHE1) and 5 (NHE5) in dorsal root ganglion (DRG) and dorsal spinal cord as well as its possible role in three inflammatory nociception tests were determined. Local peripheral ipsilateral, but not contralateral, administration of NHE inhibitors 5-(N,N-dimethyl)amiloride hydrochloride (DMA, 0.3-30 μM/paw), 5-(N-ethyl-N-isopropyl)amiloride (EIPA, 0.3-30 μM/paw) and amiloride (0.1-10 μM/paw) significantly increased flinching but not licking behavior in the capsaicin and 5-HT tests. Moreover, DMA and EIPA (0.03-30 μM/paw) as well as amiloride (0.1-1 μM/paw) augmented, in a dose-dependent manner, 0.5% formalin-induced flinching behavior during phase II but not during phase I. Reverse transcription-polymerase chain reaction showed the expression of NHE1 and NHE5 in DRG and dorsal spinal cord. Western blot analysis confirmed the presence of NHE1 in DRG and spinal cord. Moreover, NHE5 was expressed in dorsal spinal cord, but not in DRG where a 45 kDa truncated isoform of NHE5 was identified. Collectively, these data suggest that NHE1, but not NHE5, plays an important role reducing inflammatory pain in rats.
AB - mRNA and protein presence of Na+/H+ exchanger (NHE) 1 (NHE1) and 5 (NHE5) in dorsal root ganglion (DRG) and dorsal spinal cord as well as its possible role in three inflammatory nociception tests were determined. Local peripheral ipsilateral, but not contralateral, administration of NHE inhibitors 5-(N,N-dimethyl)amiloride hydrochloride (DMA, 0.3-30 μM/paw), 5-(N-ethyl-N-isopropyl)amiloride (EIPA, 0.3-30 μM/paw) and amiloride (0.1-10 μM/paw) significantly increased flinching but not licking behavior in the capsaicin and 5-HT tests. Moreover, DMA and EIPA (0.03-30 μM/paw) as well as amiloride (0.1-1 μM/paw) augmented, in a dose-dependent manner, 0.5% formalin-induced flinching behavior during phase II but not during phase I. Reverse transcription-polymerase chain reaction showed the expression of NHE1 and NHE5 in DRG and dorsal spinal cord. Western blot analysis confirmed the presence of NHE1 in DRG and spinal cord. Moreover, NHE5 was expressed in dorsal spinal cord, but not in DRG where a 45 kDa truncated isoform of NHE5 was identified. Collectively, these data suggest that NHE1, but not NHE5, plays an important role reducing inflammatory pain in rats.
KW - NHE inhibitors
KW - Na/H exchanger
KW - amiloride
KW - pain
KW - peripheral sensitization
UR - http://www.scopus.com/inward/record.url?scp=64149126259&partnerID=8YFLogxK
U2 - 10.1016/j.neuroscience.2009.02.033
DO - 10.1016/j.neuroscience.2009.02.033
M3 - Artículo
C2 - 19248819
SN - 0306-4522
VL - 160
SP - 156
EP - 164
JO - Neuroscience
JF - Neuroscience
IS - 1
ER -