Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding

Adebiyi A. Adeniran, NADIA ANGÈLICA FERNANDEZ SANTOS, Jorge J. Rodríguez-Rojas, Nancy Treviño Garza, Herón Huerta-Jiménez, Pedro C. Mis-Ávila, Wilbert A. Pérez-Pech, Luis M. Hernández-Triana, Mario Alberto Rodríguez Pérez

Research output: Contribution to specialist publicationArticle

Abstract

Leishmaniasis, a vector‐borne disease transmitted to humans through the bite of phlebotomine sand flies, is of public health significance in southeastern Mexico. Active and continuous monitoring of vectors is an important aspect of disease control for the prediction of potential outbreaks. Thus, the correct identification of vectors is paramount in this regard. In this study, we employed DNA barcoding as a tool for identifying phlebotomine sand flies collected in localized cutaneous leishmaniasis endemic areas of Quintana Roo, Mexico. Specimens were collected using CDC light and Shannon traps as part of the Mexican Ministry of Health surveillance program. DNA extraction was carried out using a nondestructive protocol, and morphological identification based on taxonomic keys was conducted on slide‐mounted specimens. Molecular taxonomic resolution using the 658‐bp fragment of the mitochondrial cytochrome
Original languageEnglish
Pages13543-13554
Number of pages12
Volume9
No23
Specialist publicationEcology and Evolution
StatePublished - 21 Nov 2019

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