TY - JOUR
T1 - Identification of peptides presented by HLA class I molecules on cervical cancer cells with HPV-18 infection
AU - Monroy-García, Alberto
AU - Ortíz-Navarrete, Vianney Francisco
AU - Mora-García, María De Lourdes
AU - Flores-Borja, Fabián
AU - Diaz-Quiñonez, Alberto
AU - Isibasi-Araujo, Armando
AU - Trejo-Becerril, Catalina
AU - Chacón-Salinas, Rommel
AU - Hernández-Montes, Jorge
AU - Granados-Arreola, Julio
AU - De Leo, Claudia
AU - Weiss-Steider, Benny
N1 - Funding Information:
We are grateful to Ph.D. Gerd Moldenhauer, at the German Cancer Research Center Heidelberg, for supplying the antibody W6/32. We also thank Dr Alejandro Garcı́a-Carrancá, Instituto de Investigaciones Biomédicas, UNAM and Dr Jaime Mas Oliva, Instituto de Fisiologı́a Celular, UNAM, for helpful discussions. Dr Jorge Arellano Blanco, Unidad de Pediatrı́a at the Centro Médico Nacional Siglo XXI and M en C. Miriam Guido, Instituto de Investigaciones Biomédicas, UNAM, for HLA and HPV typing for the INBL cell line. Finally we also thank Dr José Moreno Rodrı́guez for support in the FACScan analysis, Ranulfo Pedraza and José Chavarrı́a for excellent technical assistance. This work was supported by DGAPA-UNAM grants: PAPIIT-IN206993 and IN216597; CONACyT grant 25443-M; PADEP-030302, 030349 and 030368; and PUIS/FES-Zaragoza scholarships.
PY - 1999/4/15
Y1 - 1999/4/15
N2 - In this work we eluted peptides from purified class I MHC molecules, isolated from a novel human cervical carcinoma cell line (INBL), generated in our laboratory and positive for HPV-18 infection. A fraction of these peptides was capable of stimulating T lymphocytes obtained from a donor matched for HLA-Cw4 and who was also HPV-18+. Direct N-terminal Edman degradation of these peptides, revealed the sequence (XQFPIFLQF) that matched 85% with the sequence NVFPIFLQM localized in between the 54 and 62 residues of the HPV-18 L1 protein. After stimulation with the synthetic peptide NVFPIFLQM, T lymphocytes from the donor were capable to lyse INBL cells. Our results provide evidence of the existence of naturally occurring viral epitopes presented on cervical cancer cells by the HLA-Cw4 allele, that could be useful for immunotherapy on this type of patient.
AB - In this work we eluted peptides from purified class I MHC molecules, isolated from a novel human cervical carcinoma cell line (INBL), generated in our laboratory and positive for HPV-18 infection. A fraction of these peptides was capable of stimulating T lymphocytes obtained from a donor matched for HLA-Cw4 and who was also HPV-18+. Direct N-terminal Edman degradation of these peptides, revealed the sequence (XQFPIFLQF) that matched 85% with the sequence NVFPIFLQM localized in between the 54 and 62 residues of the HPV-18 L1 protein. After stimulation with the synthetic peptide NVFPIFLQM, T lymphocytes from the donor were capable to lyse INBL cells. Our results provide evidence of the existence of naturally occurring viral epitopes presented on cervical cancer cells by the HLA-Cw4 allele, that could be useful for immunotherapy on this type of patient.
KW - Cervical cancer cells
KW - HLA class I bound peptides
KW - Human papillomavirus
KW - Viral antigens
UR - http://www.scopus.com/inward/record.url?scp=0345280016&partnerID=8YFLogxK
U2 - 10.1016/S0165-2478(98)00188-6
DO - 10.1016/S0165-2478(98)00188-6
M3 - Artículo
SN - 0165-2478
VL - 67
SP - 167
EP - 177
JO - Immunology Letters
JF - Immunology Letters
IS - 3
ER -