TY - JOUR
T1 - Identification of Bacillus megaterium and Microbacterium liquefaciens genes involved in metal resistance and metal removal
AU - Fierros-Romer, Grisel
AU - Gómez-Ramírez, Marlenne
AU - Arenas-Isaac, Ginesa E.
AU - Pless, Reynaldo C.
AU - Rojas-Avelizapa, Norma G.
N1 - Publisher Copyright:
© 2015, National Research Council of Canada. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Bacillus megaterium MNSH1-9K-1 and Microbacterium liquefaciens MNSH2-PHGII-2, 2 nickel- and vanadiumresistant bacteria from mine tailings located in Guanajuato, Mexico, are shown to have the ability to remove 33.1% and 17.8% of Ni, respectively, and 50.8% and 14.0% of V, respectively, from spent petrochemical catalysts containing 428 ± 30 mg·kg−1 Ni and 2165 ± 77 mg·kg−1 V. In these strains, several Ni resistance determinants were detected by conventional PCR. The nccA (nickel–cobalt–cadmium resistance) was found for the first time in B. megaterium. In M. liquefaciens, the above gene as well as the czcD gene (cobalt–zinc–cadmium resistance) and a high-affinity nickel transporter were detected for the first time. This study characterizes the resistance of M. liquefaciens and B. megaterium to Ni through the expression of genes conferring metal resistance.
AB - Bacillus megaterium MNSH1-9K-1 and Microbacterium liquefaciens MNSH2-PHGII-2, 2 nickel- and vanadiumresistant bacteria from mine tailings located in Guanajuato, Mexico, are shown to have the ability to remove 33.1% and 17.8% of Ni, respectively, and 50.8% and 14.0% of V, respectively, from spent petrochemical catalysts containing 428 ± 30 mg·kg−1 Ni and 2165 ± 77 mg·kg−1 V. In these strains, several Ni resistance determinants were detected by conventional PCR. The nccA (nickel–cobalt–cadmium resistance) was found for the first time in B. megaterium. In M. liquefaciens, the above gene as well as the czcD gene (cobalt–zinc–cadmium resistance) and a high-affinity nickel transporter were detected for the first time. This study characterizes the resistance of M. liquefaciens and B. megaterium to Ni through the expression of genes conferring metal resistance.
KW - Bacillus megaterium
KW - Metal resistance
KW - Microbacterium liquefaciens
KW - Ni and V removal
KW - Spent catalyst
UR - http://www.scopus.com/inward/record.url?scp=84971492456&partnerID=8YFLogxK
U2 - 10.1139/cjm-2015-0507
DO - 10.1139/cjm-2015-0507
M3 - Artículo
C2 - 27210016
SN - 0008-4166
VL - 62
SP - 505
EP - 513
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 6
ER -