TY - JOUR
T1 - Identification by Q-PCR of Trypanosoma cruzi lineage and determination of blood meal sources in triatomine gut samples in México
AU - Ibáñez-Cervantes, Gabriela
AU - Martínez-Ibarra, Alejandro
AU - Nogueda-Torres, Benjamín
AU - López-Orduña, Eduardo
AU - Alonso, Ana L.
AU - Perea, Cynthia
AU - Maldonado, Teresa
AU - Hernández, José Manuel
AU - León-Avila, Gloria
N1 - Funding Information:
We thank Dr. Jose Luis Rosales for the Y clone culture. This work was supported by grant 20100726 from SIP-IPN , by the grant 90839 from CONACyT México and by CONACyT and PIFI scholarships granted to the first author. We thank Dr. Ernesto Ramírez and Juan Carlos del Mazo Lopez from InDRE for the help in using the Agilent Technologies Software 2100 and Dr. Gerardo Zuñiga for the advice in the Phylogenetic analysis.
PY - 2013/2
Y1 - 2013/2
N2 - Triatomine vectors were collected on human dwellings in Michoacán México. Blood meal sources were identified by real time polymerase chain reaction (Q-PCR) using DNA extracted from triatomine guts. The assay was performed with one only specific primer set to amplify a fragment of the mitochondrial 12S ribosomal gene from vertebrate species. Also Trypanosoma cruzi parasites were detected in triatomine gut samples by microscopy and the positive infection was tested in mice. In addition T. cruzi discrete taxonomic units (DTUs) were identified by Q-PCR with two sets of primers that amplify the mini-circle region (miniexon) and 18S ribosomal mitochondrial gene. The sequences obtained from 18S ribosomal gene amplifications confirmed the presence of T. cruzi I and II lineages, and provide evidence of the presence of lineage TcIII and TcIV.
AB - Triatomine vectors were collected on human dwellings in Michoacán México. Blood meal sources were identified by real time polymerase chain reaction (Q-PCR) using DNA extracted from triatomine guts. The assay was performed with one only specific primer set to amplify a fragment of the mitochondrial 12S ribosomal gene from vertebrate species. Also Trypanosoma cruzi parasites were detected in triatomine gut samples by microscopy and the positive infection was tested in mice. In addition T. cruzi discrete taxonomic units (DTUs) were identified by Q-PCR with two sets of primers that amplify the mini-circle region (miniexon) and 18S ribosomal mitochondrial gene. The sequences obtained from 18S ribosomal gene amplifications confirmed the presence of T. cruzi I and II lineages, and provide evidence of the presence of lineage TcIII and TcIV.
KW - Chagas disease
KW - DTU
KW - Feeding sources
KW - Q-PCR
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=84869886836&partnerID=8YFLogxK
U2 - 10.1016/j.parint.2012.09.003
DO - 10.1016/j.parint.2012.09.003
M3 - Artículo
C2 - 22995149
AN - SCOPUS:84869886836
SN - 1383-5769
VL - 62
SP - 36
EP - 43
JO - Parasitology International
JF - Parasitology International
IS - 1
ER -
