TY - JOUR
T1 - Identification and expression pattern of a new carotenoid cleavage dioxygenase gene member from Bixa orellana
AU - Rodríguez-Ávila, N. L.
AU - Narváez-Zapata, J. A.
AU - Ramírez-Benítez, J. E.
AU - Aguilar-Espinosa, M. L.
AU - Rivera-Madrid, R.
N1 - Funding Information:
This work was supported by the International Foundation for Science (IFS) F/2932-3 and by the Consejo Nacional de Ciencia y Tecnología (CONACYT) 46541, 98508; and UC Mexus-CONACYT. NLRA was supported by a CONACYT PhD grant (no. 196432). We would like to thank Dr Yumi Nakazawa for her technical assistance provided in the RACE analysis. Thanks also to the two anonymous reviewers for improving the manuscript.
PY - 2011/11
Y1 - 2011/11
N2 - Carotenoid cleavage dioxygenases (CCDs) are a class of enzymes involved in the biosynthesis of a broad diversity of secondary metabolites known as apocarotenoids. In plants, CCDs are part of a genetic family with members which cleave specific double bonds of carotenoid molecules. CCDs are involved in the production of diverse and important metabolites such as vitamin A and abscisic acid (ABA). Bixa orellana L. is the main source of the natural pigment annatto or bixin, an apocarotenoid accumulated in large quantities in its seeds. Bixin biosynthesis has been studied and the involvement of a CCD has been confirmed in vitro. However, the CCD genes involved in the biosynthesis of the wide variety of apocarotenoids found in this plant have not been well documented. In this study, a new CCD1 gene member (BoCCD1) was identified and its expression was charaterized in different plant tissues of B. orellana plantlets and adult plants. The BoCCD1 sequence showed high homology with plant CCD1s involved mainly in the cleavage of carotenoids in several sites to generate multiple apocarotenoid products. Here, the expression profiles of the BoCCD1 gene were analysed and discussed in relation to total carotenoids and other important apocarotenoids such as bixin.
AB - Carotenoid cleavage dioxygenases (CCDs) are a class of enzymes involved in the biosynthesis of a broad diversity of secondary metabolites known as apocarotenoids. In plants, CCDs are part of a genetic family with members which cleave specific double bonds of carotenoid molecules. CCDs are involved in the production of diverse and important metabolites such as vitamin A and abscisic acid (ABA). Bixa orellana L. is the main source of the natural pigment annatto or bixin, an apocarotenoid accumulated in large quantities in its seeds. Bixin biosynthesis has been studied and the involvement of a CCD has been confirmed in vitro. However, the CCD genes involved in the biosynthesis of the wide variety of apocarotenoids found in this plant have not been well documented. In this study, a new CCD1 gene member (BoCCD1) was identified and its expression was charaterized in different plant tissues of B. orellana plantlets and adult plants. The BoCCD1 sequence showed high homology with plant CCD1s involved mainly in the cleavage of carotenoids in several sites to generate multiple apocarotenoid products. Here, the expression profiles of the BoCCD1 gene were analysed and discussed in relation to total carotenoids and other important apocarotenoids such as bixin.
KW - Annatto
KW - Bixa orellana
KW - CCD1
KW - apocarotenoids
KW - bixin
KW - carotenoid cleavage dioxygenase
KW - in situ RT-PCR
KW - pigments
UR - http://www.scopus.com/inward/record.url?scp=82255183883&partnerID=8YFLogxK
U2 - 10.1093/jxb/err201
DO - 10.1093/jxb/err201
M3 - Artículo de revisión
C2 - 21813796
SN - 0022-0957
VL - 62
SP - 5385
EP - 5395
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 15
ER -