Identification and analysis of the u6 small nuclear RNA gene from Entamoeba histolytica

Raúl Miranda; Luis M. Salgado; Rosana Sánchez-López; Alejandro Alagón; Paul M. Lizardi

doi:10.1016/S0378-1119(96)00397-6

Identification and analysis of the u6 small nuclear RNA gene from Entamoeba histolytica

Raúl Miranda, Luis M. Salgado, Rosana Sánchez-López, Alejandro Alagón, Paul M. Lizardi

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Among the small nuclear RNAs (snRNAs) involved in the spliceosomal processing of pre-mRNA, U6 is the most conserved. As a first evidence for the presence of the splicing machinery in the amitochondrial protozoan Entamoeba histolytica (Eh), we have cloned the u6 snRNA gene. We find that in this organism u6 is a single copy gene that is transcribed as a poly(A)^- RNA molecule of approximately 105 nucleotides. We have mapped the 5' end of the U6 snRNA transcript, and identified typical elements of a putative polymerase III promoter. This is the first snRNA gene reported in Eh. Sequence analysis indicates that this gene contains all the conserved nucleotides known to be important for U6 snRNA function. These results, in conjunction with the earlier finding of genes that contain pre-mRNA introns, suggest that Eh has a functional spliceosomal complex.

Original language	English
Pages (from-to)	37-42
Number of pages	6
Journal	Gene
Volume	180
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(96)00397-6
State	Published - 21 Nov 1996
Externally published	Yes

Keywords

Amoeba
Intron
Phylogenetics
Splicing

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10.1016/S0378-1119(96)00397-6

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title = "Identification and analysis of the u6 small nuclear RNA gene from Entamoeba histolytica",

abstract = "Among the small nuclear RNAs (snRNAs) involved in the spliceosomal processing of pre-mRNA, U6 is the most conserved. As a first evidence for the presence of the splicing machinery in the amitochondrial protozoan Entamoeba histolytica (Eh), we have cloned the u6 snRNA gene. We find that in this organism u6 is a single copy gene that is transcribed as a poly(A)- RNA molecule of approximately 105 nucleotides. We have mapped the 5' end of the U6 snRNA transcript, and identified typical elements of a putative polymerase III promoter. This is the first snRNA gene reported in Eh. Sequence analysis indicates that this gene contains all the conserved nucleotides known to be important for U6 snRNA function. These results, in conjunction with the earlier finding of genes that contain pre-mRNA introns, suggest that Eh has a functional spliceosomal complex.",

keywords = "Amoeba, Intron, Phylogenetics, Splicing",

author = "Ra{\'u}l Miranda and Salgado, {Luis M.} and Rosana S{\'a}nchez-L{\'o}pez and Alejandro Alag{\'o}n and Lizardi, {Paul M.}",

note = "Funding Information: We thank Dr. Alan M. Lambowitz for critical reading of the manuscript, and Alejandro Olvera, Felipe Olvera and Ren6 Hernfindez for their technical assistance. This work was supported by grants from DGAPA-UNAM, the Commission of the European Communities (ClI*-CT92-0017) and the Howard Hughes Medical Institute (75191-527103). P.M.L. is an International Research Scholar of the Howard Hughes Medical Institute.",

year = "1996",

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doi = "10.1016/S0378-1119(96)00397-6",

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T1 - Identification and analysis of the u6 small nuclear RNA gene from Entamoeba histolytica

AU - Miranda, Raúl

AU - Salgado, Luis M.

AU - Sánchez-López, Rosana

AU - Alagón, Alejandro

AU - Lizardi, Paul M.

N1 - Funding Information: We thank Dr. Alan M. Lambowitz for critical reading of the manuscript, and Alejandro Olvera, Felipe Olvera and Ren6 Hernfindez for their technical assistance. This work was supported by grants from DGAPA-UNAM, the Commission of the European Communities (ClI*-CT92-0017) and the Howard Hughes Medical Institute (75191-527103). P.M.L. is an International Research Scholar of the Howard Hughes Medical Institute.

PY - 1996/11/21

Y1 - 1996/11/21

N2 - Among the small nuclear RNAs (snRNAs) involved in the spliceosomal processing of pre-mRNA, U6 is the most conserved. As a first evidence for the presence of the splicing machinery in the amitochondrial protozoan Entamoeba histolytica (Eh), we have cloned the u6 snRNA gene. We find that in this organism u6 is a single copy gene that is transcribed as a poly(A)- RNA molecule of approximately 105 nucleotides. We have mapped the 5' end of the U6 snRNA transcript, and identified typical elements of a putative polymerase III promoter. This is the first snRNA gene reported in Eh. Sequence analysis indicates that this gene contains all the conserved nucleotides known to be important for U6 snRNA function. These results, in conjunction with the earlier finding of genes that contain pre-mRNA introns, suggest that Eh has a functional spliceosomal complex.

AB - Among the small nuclear RNAs (snRNAs) involved in the spliceosomal processing of pre-mRNA, U6 is the most conserved. As a first evidence for the presence of the splicing machinery in the amitochondrial protozoan Entamoeba histolytica (Eh), we have cloned the u6 snRNA gene. We find that in this organism u6 is a single copy gene that is transcribed as a poly(A)- RNA molecule of approximately 105 nucleotides. We have mapped the 5' end of the U6 snRNA transcript, and identified typical elements of a putative polymerase III promoter. This is the first snRNA gene reported in Eh. Sequence analysis indicates that this gene contains all the conserved nucleotides known to be important for U6 snRNA function. These results, in conjunction with the earlier finding of genes that contain pre-mRNA introns, suggest that Eh has a functional spliceosomal complex.

KW - Amoeba

KW - Intron

KW - Phylogenetics

KW - Splicing

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SP - 37

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JO - Gene

JF - Gene

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ER -

Identification and analysis of the u6 small nuclear RNA gene from Entamoeba histolytica

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Keywords

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