TY - JOUR
T1 - Hypocholesterolemic Activity from the Leaf Extracts of Cnidoscolus chayamansa
AU - Miranda-Velasquez, Lylia
AU - Oranday-Cardenas, Azucena
AU - Lozano-Garza, Hector
AU - Rivas-Morales, Catalina
AU - Chamorro-Cevallos, German
AU - Cruz-Vega, Delia Elva
PY - 2010/12
Y1 - 2010/12
N2 - The aim of this study was to determine the hypocholesterolemic activity of Cnidoscolus chayamansa. In an in vivo model, high-cholesterol diet administered to mice Balb/c induced hypercholesterolemia. Three extracts from Cnidoscolus chayamansa (ethanol, methanol and an aqueous extract) were tested on hypercholesterolemic mice. Active extracts were assessed against the in vitro inhibitory activity of the same three extracts on the HMG-CoA reductase enzyme by using Vero cells. The specific chemical groups present in the phytochemical extracts were also determined. Only the aqueous extract (at either doses employed) showed a significant cholesterol reduction (27.9 and 31.1%, for 50 and 100 mg kg-1, respectively P<0.01). The extract did not inhibit the HMG-CoA reductase enzyme, suggesting that its compounds act at another level in cholesterol metabolism. Reactions to secondary metabolites indicate the presence of alkaloids in the aqueous and ethanol extracts and phenol hydroxyls in the ethanol and methanol extracts.
AB - The aim of this study was to determine the hypocholesterolemic activity of Cnidoscolus chayamansa. In an in vivo model, high-cholesterol diet administered to mice Balb/c induced hypercholesterolemia. Three extracts from Cnidoscolus chayamansa (ethanol, methanol and an aqueous extract) were tested on hypercholesterolemic mice. Active extracts were assessed against the in vitro inhibitory activity of the same three extracts on the HMG-CoA reductase enzyme by using Vero cells. The specific chemical groups present in the phytochemical extracts were also determined. Only the aqueous extract (at either doses employed) showed a significant cholesterol reduction (27.9 and 31.1%, for 50 and 100 mg kg-1, respectively P<0.01). The extract did not inhibit the HMG-CoA reductase enzyme, suggesting that its compounds act at another level in cholesterol metabolism. Reactions to secondary metabolites indicate the presence of alkaloids in the aqueous and ethanol extracts and phenol hydroxyls in the ethanol and methanol extracts.
KW - Cholesterol
KW - Cnidoscolus chayamansa
KW - HMG-CoA reductase
KW - Hypocholesterolemic
KW - Vero cells
UR - http://www.scopus.com/inward/record.url?scp=78650217586&partnerID=8YFLogxK
U2 - 10.1007/s11130-010-0202-4
DO - 10.1007/s11130-010-0202-4
M3 - Artículo
SN - 0921-9668
VL - 65
SP - 392
EP - 395
JO - Plant Foods for Human Nutrition
JF - Plant Foods for Human Nutrition
IS - 4
ER -