TY - JOUR
T1 - Hippocampus and amygdala neurotoxicity produced by systemic lidocaine in adult rats
AU - Blas-Valdivia, Vanessa
AU - Cano-Europa, Edgar
AU - Hernández-García, Adelaida
AU - Ortiz-Butrón, Rocio
N1 - Funding Information:
This study was partially supported by CONACyT grants 53227 and SIP-IPN 20060119. R.O-B is fellow of EDI and SNI, México, D.F. We thank Sergio Lopez Jose and Dr. Ellis Glazier who edited this English-language manuscript.
PY - 2007/8/2
Y1 - 2007/8/2
N2 - There is evidence that using lidocaine-treated cellular culture produces cell damage. However, there are no studies in vivo demonstrating the potential injurious effect of lidocaine on the central nervous system. Therefore, the aim of our study was to investigate if lidocaine is involved in neuronal damage in the CA3 hippocampus and amygdala regions when using a single subconvulsive or a convulsive lidocaine dose. Two-month-old male Wistar rats (57) were used. The animals were randomly assigned to one of three groups. Group I received 0.9% saline ip (n = 9), group II received a single lidocaine dose of 60 mg/kg (n = 18), and group III received 90 mg/kg ip (n = 12). At day 2, 7, and 10 after the dosing, three to six rats per group were sacrificed. The brains of the rats were removed and were embedded in paraffin. Coronal cuts of 7 μm were made. Each brain section was stained with cresyl-eosin. We evaluated the number of normal and abnormal neurons in the hippocampal CA3 (pyramidal) and basolateral amygdala (large and medium neurons) regions in a 10,000 μm2 section. To explore an association between lidocaine-induced seizure and neuronal damage, diazepam was used (10 mg/kg ig) as an anticonvulsant two hours before a 90 mg/kg dose of lidocaine. Lidocaine causes a morphological neuronal alteration in the CA3 hippocampal region and the basolateral amygdala and possibly an inhibition-excitation imbalance. Diazepam prevents lidocaine-induced seizures, but not neuronal damage in brain structures. Interaction of lidocaine with the membrane components produces disrupted Ca+ 2 homeostasis and causes neuronal damage. Moreover, it is possible that lidocaine or its metabolites could actively participate in the neuronal damage observed.
AB - There is evidence that using lidocaine-treated cellular culture produces cell damage. However, there are no studies in vivo demonstrating the potential injurious effect of lidocaine on the central nervous system. Therefore, the aim of our study was to investigate if lidocaine is involved in neuronal damage in the CA3 hippocampus and amygdala regions when using a single subconvulsive or a convulsive lidocaine dose. Two-month-old male Wistar rats (57) were used. The animals were randomly assigned to one of three groups. Group I received 0.9% saline ip (n = 9), group II received a single lidocaine dose of 60 mg/kg (n = 18), and group III received 90 mg/kg ip (n = 12). At day 2, 7, and 10 after the dosing, three to six rats per group were sacrificed. The brains of the rats were removed and were embedded in paraffin. Coronal cuts of 7 μm were made. Each brain section was stained with cresyl-eosin. We evaluated the number of normal and abnormal neurons in the hippocampal CA3 (pyramidal) and basolateral amygdala (large and medium neurons) regions in a 10,000 μm2 section. To explore an association between lidocaine-induced seizure and neuronal damage, diazepam was used (10 mg/kg ig) as an anticonvulsant two hours before a 90 mg/kg dose of lidocaine. Lidocaine causes a morphological neuronal alteration in the CA3 hippocampal region and the basolateral amygdala and possibly an inhibition-excitation imbalance. Diazepam prevents lidocaine-induced seizures, but not neuronal damage in brain structures. Interaction of lidocaine with the membrane components produces disrupted Ca+ 2 homeostasis and causes neuronal damage. Moreover, it is possible that lidocaine or its metabolites could actively participate in the neuronal damage observed.
KW - Lidocaine
KW - Limbic system
KW - Neurotoxicity
UR - http://www.scopus.com/inward/record.url?scp=34547987690&partnerID=8YFLogxK
U2 - 10.1016/j.lfs.2007.07.007
DO - 10.1016/j.lfs.2007.07.007
M3 - Artículo
C2 - 17689565
SN - 0024-3205
VL - 81
SP - 691
EP - 694
JO - Life Sciences
JF - Life Sciences
IS - 8
ER -