TY - JOUR
T1 - High-performance liquid chromatography assay for indorenate in pharmaceutical powders
AU - Villalobos-Hernandez, J. R.
AU - Arenas-Hernandez, R.
AU - Villafuerte-Robles, L.
PY - 2001
Y1 - 2001
N2 - A high-performance liquid chromatographic (HPLC) method for quantification of indorenate admixed of pharmaceutical excipients (Pharmatose DCL 21 Povidone USP and Helmcel 200) is described. Indorenate was extracted from the mixtures using a mobile phase composed of acetonitrile and a sodium acetate buffer solution 0.1 M (63:37) and separated from other dissolved components by ion supression-HPLC. The method was standardized using a C-18 column (250 mm × 4.8 mm, i.d., 5 μm). The photometric detector was fixed at 228 or 272 nm depending on the admixed excipients. Validation parameters included linearity, precision, accuracy, reproducibility, and specificity. The method was specific, selective, and capable to distinguish indorenate from their degradation products and the antihypertensive pelanserine.
AB - A high-performance liquid chromatographic (HPLC) method for quantification of indorenate admixed of pharmaceutical excipients (Pharmatose DCL 21 Povidone USP and Helmcel 200) is described. Indorenate was extracted from the mixtures using a mobile phase composed of acetonitrile and a sodium acetate buffer solution 0.1 M (63:37) and separated from other dissolved components by ion supression-HPLC. The method was standardized using a C-18 column (250 mm × 4.8 mm, i.d., 5 μm). The photometric detector was fixed at 228 or 272 nm depending on the admixed excipients. Validation parameters included linearity, precision, accuracy, reproducibility, and specificity. The method was specific, selective, and capable to distinguish indorenate from their degradation products and the antihypertensive pelanserine.
UR - http://www.scopus.com/inward/record.url?scp=0035123757&partnerID=8YFLogxK
U2 - 10.1081/DDC-100000126
DO - 10.1081/DDC-100000126
M3 - Artículo
SN - 0363-9045
VL - 27
SP - 39
EP - 45
JO - Drug Development and Industrial Pharmacy
JF - Drug Development and Industrial Pharmacy
IS - 1
ER -