TY - JOUR
T1 - Heterologous expression of manganese peroxidase in Aspergillus niger and its effect on phenanthrene removal from soil
AU - Cortés-Espinosa, Diana V.
AU - Absalón, Ángel E.
AU - Sanchez, Noé
AU - Loera, Octavio
AU - Rodríguez-Vázquez, Refugio
AU - Fernández, Francisco J.
PY - 2012/1
Y1 - 2012/1
N2 - A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.
AB - A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.
KW - Aspergillus niger
KW - Heterologous expression
KW - Manganese peroxidase
KW - Phenanthrene
KW - Soil remediation
UR - http://www.scopus.com/inward/record.url?scp=84856508014&partnerID=8YFLogxK
U2 - 10.1159/000331563
DO - 10.1159/000331563
M3 - Artículo
C2 - 22286039
SN - 1464-1801
VL - 21
SP - 120
EP - 129
JO - Journal of Molecular Microbiology and Biotechnology
JF - Journal of Molecular Microbiology and Biotechnology
IS - 3-4
ER -