Haemophilus influenzae triggers autophagy in HEp-2 cells

María del Rosario Espinoza-Mellado; Carolina Reyes-Picaso; Miriam S. Garcés-Pérez; Cynthia V. Jardón-Serrano; Edgar O. López-Villegas; Silvia Giono-Cerezo

doi:10.1007/s00203-015-1167-3

Haemophilus influenzae triggers autophagy in HEp-2 cells

María del Rosario Espinoza-Mellado, Carolina Reyes-Picaso, Miriam S. Garcés-Pérez, Cynthia V. Jardón-Serrano, Edgar O. López-Villegas, Silvia Giono-Cerezo

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (HEp-2). We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, containing <50 kDa proteins, of both strains. Ultrastructural studies demonstrate vacuoles with a double membrane and/or membrane material inside, showing similar features to those of autophagic vacuoles. Together, our findings demonstrate that H. influenzae strains and their supernatants trigger an autophagic process.

Original language	English
Pages (from-to)	199-204
Number of pages	6
Journal	Archives of Microbiology
Volume	198
Issue number	2
DOIs	https://doi.org/10.1007/s00203-015-1167-3
State	Published - 1 Mar 2016

Keywords

Autophagic vacuoles
Autophagy
Haemophilus influenzae
LC3B protein

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10.1007/s00203-015-1167-3

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title = " Haemophilus influenzae triggers autophagy in HEp-2 cells",

abstract = "The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (HEp-2). We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, containing <50 kDa proteins, of both strains. Ultrastructural studies demonstrate vacuoles with a double membrane and/or membrane material inside, showing similar features to those of autophagic vacuoles. Together, our findings demonstrate that H. influenzae strains and their supernatants trigger an autophagic process.",

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doi = "10.1007/s00203-015-1167-3",

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T1 - Haemophilus influenzae triggers autophagy in HEp-2 cells

AU - Espinoza-Mellado, María del Rosario

AU - Reyes-Picaso, Carolina

AU - Garcés-Pérez, Miriam S.

AU - Jardón-Serrano, Cynthia V.

AU - López-Villegas, Edgar O.

AU - Giono-Cerezo, Silvia

PY - 2016/3/1

Y1 - 2016/3/1

N2 - The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (HEp-2). We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, containing <50 kDa proteins, of both strains. Ultrastructural studies demonstrate vacuoles with a double membrane and/or membrane material inside, showing similar features to those of autophagic vacuoles. Together, our findings demonstrate that H. influenzae strains and their supernatants trigger an autophagic process.

AB - The MAP-LC3 system regulates the intracellular formation of autophagy-associated vacuoles. These vacuoles contain the LC3 protein; thus it has been utilized as a marker to identify autophagosomes. The aim of our study was to investigate whether Haemophilus influenzae strains and their supernatants could activate autophagy in human larynx carcinoma cell line (HEp-2). We demonstrate that higher expression of the LC3B-II protein was induced, particularly by nontypeable Haemophilus influenzae (NTHi) 49766 and by supernatants, containing <50 kDa proteins, of both strains. Ultrastructural studies demonstrate vacuoles with a double membrane and/or membrane material inside, showing similar features to those of autophagic vacuoles. Together, our findings demonstrate that H. influenzae strains and their supernatants trigger an autophagic process.

KW - Autophagic vacuoles

KW - Autophagy

KW - Haemophilus influenzae

KW - LC3B protein

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U2 - 10.1007/s00203-015-1167-3

DO - 10.1007/s00203-015-1167-3

M3 - Artículo

C2 - 26537814

SN - 0302-8933

VL - 198

SP - 199

EP - 204

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 2

ER -

Haemophilus influenzae triggers autophagy in HEp-2 cells

Abstract

Keywords

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