TY - JOUR
T1 - Gonadal development and biochemical composition of female crayfish Cherax quadricarinatus (Decapoda: Parastacidae) in relation to the Gonadosomatic Index at first maturation
AU - Rodríguez-González, Hervey
AU - Hernández-Llamas, Alfredo
AU - Villarreal, Humberto
AU - Saucedo, Pedro E.
AU - García-Ulloa, Manuel
AU - Rodríguez-Jaramillo, Carmen
N1 - Funding Information:
The authors are grateful to Francisco Encarnación and Ismael Valdivia for assistance during management and sampling phases. Ilie Racotta reviewed the manuscript and provided helpful criticism. The comments of two anonymous reviewers are also acknowledged. Axel Buchner helped conducting power analysis with G*Power. The authors are responsible for the analysis. The editor at CIBNOR provided assistance with improving the English text. This study was supported by a CONACYT grant U39531-Z and a CIBNOR grant AC.2.1 to Humberto Villarreal. Hervey Rodríguez is a doctoral fellow of CONACYT (144580).
PY - 2006/4/28
Y1 - 2006/4/28
N2 - Gonadal development of female crayfish Cherax quadricarinatus at first maturation (25.5 ± 3.8 g) was investigated using morphological criteria (Gonadosomatic and Hepatosomatic Indices or GSI and HSI), biochemical composition (proteins, lipids, and carbohydrates) of the gonad and hepatopancreas, histological indicators (oocyte diameter, frequency of pre-vitellogenic, vitellogenic, and post-vitellogenic oocytes), and histochemical analyses (percentage coverage of lipid and carbohydrate reserves or LI and CI). There was a negative relation between GSI and HSI (P < 0.01). Protein (P < 0.01), lipid (P < 0.01), and carbohydrate (P < 0.01) contents in the gonad were dependent on GSI. At mature stages, higher concentrations of protein and lipid reserves were observed, while carbohydrates diminished. In hepatopancreas, the lipid content decreased in relation to GSI (P < 0.05). There was no evidence that protein and carbohydrate components in the hepatopancreas varied in relation to GSI (P > 0.05). High statistical power (> 0.99 for both contents) indicates that protein and carbohydrate concentration remains relatively constant throughout gonadal development. A significant decrease in the frequency of oogonia (P < 0.01), pre-vitellogenic oocytes (P < 0.01), and primary vitellogenic oocytes (P < 0.01) was observed as maturation was reached. A mathematical inflexion point showed that there was a maximum frequency of secondary vitellogenic oocytes at 4.06 GSI. There were increases in diameter and nuclei of oocytes at all developmental stages (P < 0.05). LI increased as oocytes developed (P < 0.01). CI varied between stages (P < 0.01) and peaked at the primary vitellogenic stage. The dynamics of biochemical composition of gonad and hepatopancreas of female C. quadricarinatus was in agreement with similar studies in decapods. The usefulness of the GSI as an indicator of reproduction stage and female broodstock management is addressed.
AB - Gonadal development of female crayfish Cherax quadricarinatus at first maturation (25.5 ± 3.8 g) was investigated using morphological criteria (Gonadosomatic and Hepatosomatic Indices or GSI and HSI), biochemical composition (proteins, lipids, and carbohydrates) of the gonad and hepatopancreas, histological indicators (oocyte diameter, frequency of pre-vitellogenic, vitellogenic, and post-vitellogenic oocytes), and histochemical analyses (percentage coverage of lipid and carbohydrate reserves or LI and CI). There was a negative relation between GSI and HSI (P < 0.01). Protein (P < 0.01), lipid (P < 0.01), and carbohydrate (P < 0.01) contents in the gonad were dependent on GSI. At mature stages, higher concentrations of protein and lipid reserves were observed, while carbohydrates diminished. In hepatopancreas, the lipid content decreased in relation to GSI (P < 0.05). There was no evidence that protein and carbohydrate components in the hepatopancreas varied in relation to GSI (P > 0.05). High statistical power (> 0.99 for both contents) indicates that protein and carbohydrate concentration remains relatively constant throughout gonadal development. A significant decrease in the frequency of oogonia (P < 0.01), pre-vitellogenic oocytes (P < 0.01), and primary vitellogenic oocytes (P < 0.01) was observed as maturation was reached. A mathematical inflexion point showed that there was a maximum frequency of secondary vitellogenic oocytes at 4.06 GSI. There were increases in diameter and nuclei of oocytes at all developmental stages (P < 0.05). LI increased as oocytes developed (P < 0.01). CI varied between stages (P < 0.01) and peaked at the primary vitellogenic stage. The dynamics of biochemical composition of gonad and hepatopancreas of female C. quadricarinatus was in agreement with similar studies in decapods. The usefulness of the GSI as an indicator of reproduction stage and female broodstock management is addressed.
KW - Biochemical composition
KW - Cherax quadricarinatus
KW - Gonadal development
KW - Gonadosomatic Index
KW - Redclaw crayfish
UR - http://www.scopus.com/inward/record.url?scp=33748033398&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2005.10.020
DO - 10.1016/j.aquaculture.2005.10.020
M3 - Artículo
SN - 0044-8486
VL - 254
SP - 637
EP - 645
JO - Aquaculture
JF - Aquaculture
IS - 1-4
ER -