TY - JOUR
T1 - Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus
AU - Tello-Olea, M.
AU - Rosales-Mendoza, S.
AU - Campa-Córdova, A. I.
AU - Palestino, G.
AU - Luna-González, A.
AU - Reyes-Becerril, M.
AU - Velazquez, E.
AU - Hernandez-Adame, L.
AU - Angulo, C.
N1 - Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/1
Y1 - 2019/1
N2 - Gold nanoparticles (AuNP) stimulate immune responses in mammals but they have not been tested in species of relevance in aquaculture. In this study the immunostimulant and protective potential of orally administered AuNP against V. parahaemolyticus, the causative agent of Acute Hepatopancreatic Necrosis Disease, was determined in shrimp. Synthetized AuNP (18.57 ± 4.37 nm) were moderately dispersed with a negative ζ potential of −10.3 ± 0.208 mV (pH = 7). AuNP were administered (single dose) at 0.2, 2, and 20 μg/g feed in shrimp. Hemolymph samples were withdrawn daily for 6 days. Hemolymph or hemocytes were used to determine total hemocyte counts, immune-related enzymatic activities, and expression of immune-relevant genes. Hepatopancreas was sampled for the analysis of AuNP biodistribution and histological examination. Survival was recorded daily. No mortality or toxicity signs in hepatopancreas were found. AuNP were detected in hepatopancreas. Early (24–48 h) immunostimulation was mainly related to immune gene up-regulation. Upon a challenge with V. parahaemolyticus, survival was higher (80%) and histopathological damages were lower in shrimp treated with the 2 μg/g dose when compared to the control. Therefore orally administered AuNP are proposed as immunostimulants that protect shrimp against V. parahaemolyticus infection.
AB - Gold nanoparticles (AuNP) stimulate immune responses in mammals but they have not been tested in species of relevance in aquaculture. In this study the immunostimulant and protective potential of orally administered AuNP against V. parahaemolyticus, the causative agent of Acute Hepatopancreatic Necrosis Disease, was determined in shrimp. Synthetized AuNP (18.57 ± 4.37 nm) were moderately dispersed with a negative ζ potential of −10.3 ± 0.208 mV (pH = 7). AuNP were administered (single dose) at 0.2, 2, and 20 μg/g feed in shrimp. Hemolymph samples were withdrawn daily for 6 days. Hemolymph or hemocytes were used to determine total hemocyte counts, immune-related enzymatic activities, and expression of immune-relevant genes. Hepatopancreas was sampled for the analysis of AuNP biodistribution and histological examination. Survival was recorded daily. No mortality or toxicity signs in hepatopancreas were found. AuNP were detected in hepatopancreas. Early (24–48 h) immunostimulation was mainly related to immune gene up-regulation. Upon a challenge with V. parahaemolyticus, survival was higher (80%) and histopathological damages were lower in shrimp treated with the 2 μg/g dose when compared to the control. Therefore orally administered AuNP are proposed as immunostimulants that protect shrimp against V. parahaemolyticus infection.
KW - AHPND
KW - Acute hepatopancreatic necrosis disease
KW - Crustacean
KW - Diseases
KW - Immune response
KW - Immunostimulants
KW - Metallic nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85055658924&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2018.10.056
DO - 10.1016/j.fsi.2018.10.056
M3 - Artículo
C2 - 30368027
SN - 1050-4648
VL - 84
SP - 756
EP - 767
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -