Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus

M. Tello-Olea, S. Rosales-Mendoza, A. I. Campa-Córdova, G. Palestino, A. Luna-González, M. Reyes-Becerril, E. Velazquez, L. Hernandez-Adame, C. Angulo

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2 Citations (Scopus)

Abstract

© 2018 Elsevier Ltd Gold nanoparticles (AuNP) stimulate immune responses in mammals but they have not been tested in species of relevance in aquaculture. In this study the immunostimulant and protective potential of orally administered AuNP against V. parahaemolyticus, the causative agent of Acute Hepatopancreatic Necrosis Disease, was determined in shrimp. Synthetized AuNP (18.57 ± 4.37 nm) were moderately dispersed with a negative ζ potential of −10.3 ± 0.208 mV (pH = 7). AuNP were administered (single dose) at 0.2, 2, and 20 μg/g feed in shrimp. Hemolymph samples were withdrawn daily for 6 days. Hemolymph or hemocytes were used to determine total hemocyte counts, immune-related enzymatic activities, and expression of immune-relevant genes. Hepatopancreas was sampled for the analysis of AuNP biodistribution and histological examination. Survival was recorded daily. No mortality or toxicity signs in hepatopancreas were found. AuNP were detected in hepatopancreas. Early (24–48 h) immunostimulation was mainly related to immune gene up-regulation. Upon a challenge with V. parahaemolyticus, survival was higher (80%) and histopathological damages were lower in shrimp treated with the 2 μg/g dose when compared to the control. Therefore orally administered AuNP are proposed as immunostimulants that protect shrimp against V. parahaemolyticus infection.
Original languageAmerican English
Pages (from-to)756-767
Number of pages12
JournalFish and Shellfish Immunology
DOIs
StatePublished - 1 Jan 2019
Externally publishedYes

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Immunologic Adjuvants
Vibrio parahaemolyticus
Litopenaeus vannamei
Gold
protective effect
hepatopancreas
shrimp
gold
Genes
Nanoparticles
immunostimulants
Aquaculture
Mammals
gene
immune response
hemocytes
hemolymph
aquaculture
Toxicity
mammal

Cite this

Tello-Olea, M. ; Rosales-Mendoza, S. ; Campa-Córdova, A. I. ; Palestino, G. ; Luna-González, A. ; Reyes-Becerril, M. ; Velazquez, E. ; Hernandez-Adame, L. ; Angulo, C. / Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus. In: Fish and Shellfish Immunology. 2019 ; pp. 756-767.
@article{dd35bda4e40a4f52ad0e6d8db7e76c8f,
title = "Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus",
abstract = "{\circledC} 2018 Elsevier Ltd Gold nanoparticles (AuNP) stimulate immune responses in mammals but they have not been tested in species of relevance in aquaculture. In this study the immunostimulant and protective potential of orally administered AuNP against V. parahaemolyticus, the causative agent of Acute Hepatopancreatic Necrosis Disease, was determined in shrimp. Synthetized AuNP (18.57 ± 4.37 nm) were moderately dispersed with a negative ζ potential of −10.3 ± 0.208 mV (pH = 7). AuNP were administered (single dose) at 0.2, 2, and 20 μg/g feed in shrimp. Hemolymph samples were withdrawn daily for 6 days. Hemolymph or hemocytes were used to determine total hemocyte counts, immune-related enzymatic activities, and expression of immune-relevant genes. Hepatopancreas was sampled for the analysis of AuNP biodistribution and histological examination. Survival was recorded daily. No mortality or toxicity signs in hepatopancreas were found. AuNP were detected in hepatopancreas. Early (24–48 h) immunostimulation was mainly related to immune gene up-regulation. Upon a challenge with V. parahaemolyticus, survival was higher (80{\%}) and histopathological damages were lower in shrimp treated with the 2 μg/g dose when compared to the control. Therefore orally administered AuNP are proposed as immunostimulants that protect shrimp against V. parahaemolyticus infection.",
author = "M. Tello-Olea and S. Rosales-Mendoza and Campa-C{\'o}rdova, {A. I.} and G. Palestino and A. Luna-Gonz{\'a}lez and M. Reyes-Becerril and E. Velazquez and L. Hernandez-Adame and C. Angulo",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.fsi.2018.10.056",
language = "American English",
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journal = "Fish and Shellfish Immunology",
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Tello-Olea, M, Rosales-Mendoza, S, Campa-Córdova, AI, Palestino, G, Luna-González, A, Reyes-Becerril, M, Velazquez, E, Hernandez-Adame, L & Angulo, C 2019, 'Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus', Fish and Shellfish Immunology, pp. 756-767. https://doi.org/10.1016/j.fsi.2018.10.056

Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus. / Tello-Olea, M.; Rosales-Mendoza, S.; Campa-Córdova, A. I.; Palestino, G.; Luna-González, A.; Reyes-Becerril, M.; Velazquez, E.; Hernandez-Adame, L.; Angulo, C.

In: Fish and Shellfish Immunology, 01.01.2019, p. 756-767.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Gold nanoparticles (AuNP) exert immunostimulatory and protective effects in shrimp (Litopenaeus vannamei) against Vibrio parahaemolyticus

AU - Tello-Olea, M.

AU - Rosales-Mendoza, S.

AU - Campa-Córdova, A. I.

AU - Palestino, G.

AU - Luna-González, A.

AU - Reyes-Becerril, M.

AU - Velazquez, E.

AU - Hernandez-Adame, L.

AU - Angulo, C.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - © 2018 Elsevier Ltd Gold nanoparticles (AuNP) stimulate immune responses in mammals but they have not been tested in species of relevance in aquaculture. In this study the immunostimulant and protective potential of orally administered AuNP against V. parahaemolyticus, the causative agent of Acute Hepatopancreatic Necrosis Disease, was determined in shrimp. Synthetized AuNP (18.57 ± 4.37 nm) were moderately dispersed with a negative ζ potential of −10.3 ± 0.208 mV (pH = 7). AuNP were administered (single dose) at 0.2, 2, and 20 μg/g feed in shrimp. Hemolymph samples were withdrawn daily for 6 days. Hemolymph or hemocytes were used to determine total hemocyte counts, immune-related enzymatic activities, and expression of immune-relevant genes. Hepatopancreas was sampled for the analysis of AuNP biodistribution and histological examination. Survival was recorded daily. No mortality or toxicity signs in hepatopancreas were found. AuNP were detected in hepatopancreas. Early (24–48 h) immunostimulation was mainly related to immune gene up-regulation. Upon a challenge with V. parahaemolyticus, survival was higher (80%) and histopathological damages were lower in shrimp treated with the 2 μg/g dose when compared to the control. Therefore orally administered AuNP are proposed as immunostimulants that protect shrimp against V. parahaemolyticus infection.

AB - © 2018 Elsevier Ltd Gold nanoparticles (AuNP) stimulate immune responses in mammals but they have not been tested in species of relevance in aquaculture. In this study the immunostimulant and protective potential of orally administered AuNP against V. parahaemolyticus, the causative agent of Acute Hepatopancreatic Necrosis Disease, was determined in shrimp. Synthetized AuNP (18.57 ± 4.37 nm) were moderately dispersed with a negative ζ potential of −10.3 ± 0.208 mV (pH = 7). AuNP were administered (single dose) at 0.2, 2, and 20 μg/g feed in shrimp. Hemolymph samples were withdrawn daily for 6 days. Hemolymph or hemocytes were used to determine total hemocyte counts, immune-related enzymatic activities, and expression of immune-relevant genes. Hepatopancreas was sampled for the analysis of AuNP biodistribution and histological examination. Survival was recorded daily. No mortality or toxicity signs in hepatopancreas were found. AuNP were detected in hepatopancreas. Early (24–48 h) immunostimulation was mainly related to immune gene up-regulation. Upon a challenge with V. parahaemolyticus, survival was higher (80%) and histopathological damages were lower in shrimp treated with the 2 μg/g dose when compared to the control. Therefore orally administered AuNP are proposed as immunostimulants that protect shrimp against V. parahaemolyticus infection.

U2 - 10.1016/j.fsi.2018.10.056

DO - 10.1016/j.fsi.2018.10.056

M3 - Article

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EP - 767

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

SN - 1050-4648

ER -