TY - JOUR
T1 - Global analysis of transcriptional regulators in Staphylococcus aureus
AU - Ibarra, Jose Antonio
AU - Pérez-Rueda, Ernesto
AU - Carroll, Ronan K.
AU - Shaw, Lindsey N.
N1 - Funding Information:
This work was supported by grants IN-209511 (EP-R) from DGAPA-UNAM, 155116 (EP-R) from the National Council for Science and Technology (CONACYT) and AI080626 (LNS) from the National Institute of Allergies and Infectious Disease.
PY - 2013/2/26
Y1 - 2013/2/26
N2 - Background: Staphylococcus aureus is a widely distributed human pathogen capable of infecting almost every ecological niche of the host. As a result, it is responsible for causing many different diseases. S. aureus has a vast array of virulence determinants whose expression is modulated by an intricate regulatory network, where transcriptional factors (TFs) are the primary elements. In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus (CA-MRSA) strain USA300-FPR3757.Results: A total of 135 TFs and sigma factors were identified and classified into 36 regulatory families. From these around 43% have been experimentally characterized to date, which demonstrates the significant work still at hand to unravel the regulatory network in place for this important pathogen. A comparison of the TF repertoire of S. aureus against 1209 sequenced bacterial genomes was carried out allowing us to identify a core set of orthologous TFs for the Staphylococacceae, and also allowing us to assign potential functions to previously uncharacterized TFs. Finally, the USA300 TFs were compared to those in eleven other S. aureus strains including: Newman, COL, JH1, JH9, MW2, Mu3, Mu50, N315, RF122, MRSA252 and MSSA476. We identify conserved TFs among these strains and suggest possible regulatory interactions.Conclusions: The analysis presented herein highlights the complexity of regulatory networks in S. aureus strains, identifies key conserved TFs among the Staphylococacceae, and offers unique insights into several as yet uncharacterized TFs.
AB - Background: Staphylococcus aureus is a widely distributed human pathogen capable of infecting almost every ecological niche of the host. As a result, it is responsible for causing many different diseases. S. aureus has a vast array of virulence determinants whose expression is modulated by an intricate regulatory network, where transcriptional factors (TFs) are the primary elements. In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus (CA-MRSA) strain USA300-FPR3757.Results: A total of 135 TFs and sigma factors were identified and classified into 36 regulatory families. From these around 43% have been experimentally characterized to date, which demonstrates the significant work still at hand to unravel the regulatory network in place for this important pathogen. A comparison of the TF repertoire of S. aureus against 1209 sequenced bacterial genomes was carried out allowing us to identify a core set of orthologous TFs for the Staphylococacceae, and also allowing us to assign potential functions to previously uncharacterized TFs. Finally, the USA300 TFs were compared to those in eleven other S. aureus strains including: Newman, COL, JH1, JH9, MW2, Mu3, Mu50, N315, RF122, MRSA252 and MSSA476. We identify conserved TFs among these strains and suggest possible regulatory interactions.Conclusions: The analysis presented herein highlights the complexity of regulatory networks in S. aureus strains, identifies key conserved TFs among the Staphylococacceae, and offers unique insights into several as yet uncharacterized TFs.
KW - Firmicutes
KW - Gene evolution
KW - Staphylococacceae
KW - Transcriptional regulators
KW - Virulence
UR - http://www.scopus.com/inward/record.url?scp=84874199986&partnerID=8YFLogxK
U2 - 10.1186/1471-2164-14-126
DO - 10.1186/1471-2164-14-126
M3 - Artículo
C2 - 23442205
AN - SCOPUS:84874199986
SN - 1471-2164
VL - 14
JO - BMC Genomics
JF - BMC Genomics
IS - 1
M1 - 126
ER -