TY - JOUR
T1 - Giardia lamblia
T2 - Interleukin 6 and tumor necrosis factor-alpha release from mast cells induced through an Ig-independent pathway
AU - Muñoz-Cruz, Samira
AU - Gómez-García, Argelia
AU - Millán-Ibarra, Jarenny
AU - Giono-Cerezo, Silvia
AU - Yépez-Mulia, Lilián
N1 - Funding Information:
This work was supported by Fondo de Investigación en Salud del Instituto Mexicano del Seguro Social (Grant 2006/1A)/I/083) and Consejo Nacional de Ciencia y Tecnología (CONACYT), México (Grant 080647).
PY - 2010/11
Y1 - 2010/11
N2 - Giardia lamblia is a common cause of both acute and chronic diarrheal disease in humans worldwide. It has been shown that mast cells, IL-6 and TNF-α are substantially involved in the early control of G. lamblia infection in mice. However, no studies have yet been reported concerning the interaction between mast cell and Giardia, as well as the mast cells mediators generated in response to Giardia infection. In this study we demonstrated the direct activation of mast cells by G. lamblia live trophozoites or trophozoite-derived antigens followed by an increase in tryptase expression and a significant release of the preformed mediator histamine. In addition, parasite derived antigens increased TNF-α and de novo synthesized cytokine IL-6, at the mRNA and protein level. These results strongly suggest that mast cells might be an important source not only of IL-6 but also of TNF-α during Giardia infection, playing an important role in the outcome of the infection.
AB - Giardia lamblia is a common cause of both acute and chronic diarrheal disease in humans worldwide. It has been shown that mast cells, IL-6 and TNF-α are substantially involved in the early control of G. lamblia infection in mice. However, no studies have yet been reported concerning the interaction between mast cell and Giardia, as well as the mast cells mediators generated in response to Giardia infection. In this study we demonstrated the direct activation of mast cells by G. lamblia live trophozoites or trophozoite-derived antigens followed by an increase in tryptase expression and a significant release of the preformed mediator histamine. In addition, parasite derived antigens increased TNF-α and de novo synthesized cytokine IL-6, at the mRNA and protein level. These results strongly suggest that mast cells might be an important source not only of IL-6 but also of TNF-α during Giardia infection, playing an important role in the outcome of the infection.
KW - Giardia lamblia
KW - Interleukin 6
KW - Mast cells
KW - Tumor necrosis factor-alpha
UR - http://www.scopus.com/inward/record.url?scp=77956236903&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2010.06.013
DO - 10.1016/j.exppara.2010.06.013
M3 - Artículo
C2 - 20600000
AN - SCOPUS:77956236903
SN - 0014-4894
VL - 126
SP - 298
EP - 303
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3
ER -