TY - JOUR
T1 - Genotyping of Mexican Cryptococcus neoformans and C. gattii isolates by PCR-fingerprinting
AU - Olivares, L. R.Castañón
AU - Martínez, K. Martínez
AU - Cruz, R. M.Bermúdez
AU - Rivera, M. A.Martínez
AU - Meyer, W.
AU - Espinosa, R. A.Arreguín
AU - Martínez, R. López
AU - Ruiz Palacios Y Santos, G. M.
N1 - Funding Information:
We gratefully recognize the collaboration of Gustavo Barriga, Patricia García Zaldívar, Rosa María Cervantes Tovar, Verónica Angeles, José Luis Soto, Alfredo Carmona, Joseph Torres, Elizabeth Castañeda, Françoise Dromer, Luisa Díaz, Karl Clemons, Leonel Mendoza, Pilar Ramos and Violeta Ibarra, for their valuable academic orientation. This work was supported by the Dirección General de Asuntos del Personal Académico de la UNAM, which provided financing support for the present investigation (PAPIIT IN-215997).
PY - 2009
Y1 - 2009
N2 - Cryptococcosis in Mxico is caused by both species of the Cryptococcus species complex i.e., Cryptococcus neoformans and C. gattii. The current study was aimed to determine genetic variability of 72 Mexican clinical isolates using PCR-fingerprinting with the primer M13. PCR fingerprinting revealed 55 VNI, five VNII, three VNIII, one VNIV, two VGI, two VGII, two VGIII and two VGIV isolates among those studied. The results show that most cryptococcosis cases in Mxico are AIDS related and are caused by C. neoformans var. grubii, genotypes VNI and VNII. In addition this study revealed for the first time the presence of genotypes VNIV and VGII among Mexican clinical isolates. The present data show that all genotypes that have been described for the Cryptococcus species complex are found in Mxico, indicating a much wider geographic distribution of genotypes than previously reported. The molecular analysis of Mexican cryptococcal isolates generated PCR-fingerprinting patterns which will provide references for future typing studies to allow the integration of Mexican cryptococcal genotypes into the ongoing global genotyping study of the Cryptococcus species complex.
AB - Cryptococcosis in Mxico is caused by both species of the Cryptococcus species complex i.e., Cryptococcus neoformans and C. gattii. The current study was aimed to determine genetic variability of 72 Mexican clinical isolates using PCR-fingerprinting with the primer M13. PCR fingerprinting revealed 55 VNI, five VNII, three VNIII, one VNIV, two VGI, two VGII, two VGIII and two VGIV isolates among those studied. The results show that most cryptococcosis cases in Mxico are AIDS related and are caused by C. neoformans var. grubii, genotypes VNI and VNII. In addition this study revealed for the first time the presence of genotypes VNIV and VGII among Mexican clinical isolates. The present data show that all genotypes that have been described for the Cryptococcus species complex are found in Mxico, indicating a much wider geographic distribution of genotypes than previously reported. The molecular analysis of Mexican cryptococcal isolates generated PCR-fingerprinting patterns which will provide references for future typing studies to allow the integration of Mexican cryptococcal genotypes into the ongoing global genotyping study of the Cryptococcus species complex.
KW - Cryptococcosis
KW - Cryptococcus gattii
KW - Cryptococcus neoformans
KW - Genotyping
KW - México
KW - PCR-fi ngerprinting
UR - http://www.scopus.com/inward/record.url?scp=71049119060&partnerID=8YFLogxK
U2 - 10.3109/13693780802559031
DO - 10.3109/13693780802559031
M3 - Artículo
C2 - 19888804
AN - SCOPUS:71049119060
SN - 1369-3786
VL - 47
SP - 713
EP - 721
JO - Medical Mycology
JF - Medical Mycology
IS - 7
ER -
