TY - JOUR
T1 - Genotoxicity of aflatoxin B1 and its ammonium derivatives
AU - Marquez, Rene Marquez
AU - De Hernandez, Irma Tejada
AU - Bujaidar, Eduardo Madrigal
PY - 1995
Y1 - 1995
N2 - Aflatoxin (AF) B1 is a main contaminant in diverse agricultural products. In an attempt to reduce this problem and the hazards to human health, an AFB1 inactivating system with ammonia has been developed. In this work we evaluated the efficiency of the system in mice using micronucleus (MN) and sister chromatid exchange (SCE) analysis. Four groups of animals were fed for 8 weeks with a special diet mainly composed of maize: (1) uncontaminated; (2) uncontaminated/inactivated; (3) contaminated/inactivated; and (4) contaminated.We evaluated MN at weekly intervals in peripheral blood, and in weeks 4 and 8 SCE frequencies were quantified in bone marrow cells. The results shows that animals fed with AFB 1 contaminated/inactivated maize had a 45% lower level of induced cytogenetic damage than those animals fed with AFB 1 contaminated but not inactivated maize. A residual amount of AFB1 after the inactivating treatment and reconversion back to AFB1 in the organism may account for the remaining increased levels of SCE and MN.
AB - Aflatoxin (AF) B1 is a main contaminant in diverse agricultural products. In an attempt to reduce this problem and the hazards to human health, an AFB1 inactivating system with ammonia has been developed. In this work we evaluated the efficiency of the system in mice using micronucleus (MN) and sister chromatid exchange (SCE) analysis. Four groups of animals were fed for 8 weeks with a special diet mainly composed of maize: (1) uncontaminated; (2) uncontaminated/inactivated; (3) contaminated/inactivated; and (4) contaminated.We evaluated MN at weekly intervals in peripheral blood, and in weeks 4 and 8 SCE frequencies were quantified in bone marrow cells. The results shows that animals fed with AFB 1 contaminated/inactivated maize had a 45% lower level of induced cytogenetic damage than those animals fed with AFB 1 contaminated but not inactivated maize. A residual amount of AFB1 after the inactivating treatment and reconversion back to AFB1 in the organism may account for the remaining increased levels of SCE and MN.
KW - Ammoniation
KW - Genotoxicity
KW - Maize
KW - Micronucleus
KW - SCE
KW - aflatoxin B
UR - http://www.scopus.com/inward/record.url?scp=0028989211&partnerID=8YFLogxK
U2 - 10.1080/02652039509374325
DO - 10.1080/02652039509374325
M3 - Artículo
SN - 0265-203X
VL - 12
SP - 425
EP - 429
JO - Food Additives and Contaminants
JF - Food Additives and Contaminants
IS - 3
ER -