TY - JOUR
T1 - Genotoxic evaluation of ammonium inactivated aflatoxin B1 in mice fed with contaminated corn
AU - Márquez-Márquez, R.
AU - Madrigal-Bujaidar, E.
AU - Tejada de Hernández, I.
PY - 1993/3
Y1 - 1993/3
N2 - Aflatoxin B1 (AFB1) is a major contaminant in different agricultural products including maize. In an attempt to reduce this problem and the hazards to human health, an AFB1 inactivating system with ammonia has been developed. In this work we evaluated the efficiency of the system in mice using micronucleus (MN) and sister chromatid exchange (SCE) analysis. Four groups of animals were fed during 8 weeks with a special diet mainly composed of maize: (1) uncontaminated; (2) uncontaminated/ inactivated; (3) contaminated/inactivated; and (4) contaminated. We evaluated MN at weekly intervals in peripheral blood, and in weeks 4 and 8 SCE frequencies were quantified in bone marrow cells. The results showed that animals fed with AFB1 contaminated/inactivated maize had a 45% lower level of induced cytogenetic damage than those animals fed with AFB1 contaminated, but not inactivated maize. A residual amount of AFB1 after the inactivating treatment and the reconversion back to AFB1 in the organism may account for the remaining increased levels of SCE and MN.
AB - Aflatoxin B1 (AFB1) is a major contaminant in different agricultural products including maize. In an attempt to reduce this problem and the hazards to human health, an AFB1 inactivating system with ammonia has been developed. In this work we evaluated the efficiency of the system in mice using micronucleus (MN) and sister chromatid exchange (SCE) analysis. Four groups of animals were fed during 8 weeks with a special diet mainly composed of maize: (1) uncontaminated; (2) uncontaminated/ inactivated; (3) contaminated/inactivated; and (4) contaminated. We evaluated MN at weekly intervals in peripheral blood, and in weeks 4 and 8 SCE frequencies were quantified in bone marrow cells. The results showed that animals fed with AFB1 contaminated/inactivated maize had a 45% lower level of induced cytogenetic damage than those animals fed with AFB1 contaminated, but not inactivated maize. A residual amount of AFB1 after the inactivating treatment and the reconversion back to AFB1 in the organism may account for the remaining increased levels of SCE and MN.
KW - (Mouse
KW - ) Micronucleus
KW - Aflatoxin B
KW - Ammonia inactivation
KW - Maize
KW - Sister-chromatid exchange
UR - http://www.scopus.com/inward/record.url?scp=0027402559&partnerID=8YFLogxK
U2 - 10.1016/0165-1218(93)90112-Q
DO - 10.1016/0165-1218(93)90112-Q
M3 - Artículo
SN - 0165-1218
VL - 299
SP - 1
EP - 8
JO - Mutation Research - Genetic Toxicology
JF - Mutation Research - Genetic Toxicology
IS - 1
ER -