TY - JOUR
T1 - Genetic expression profiles and chromosomal alterations in sporadic breast cancer in Mexican women
AU - Valladares, Adán
AU - Hernández, Normand García
AU - Gómez, Fabio Salamanca
AU - Curiel-Quezada, Everardo
AU - Madrigal-Bujaidar, Eduardo
AU - Vergara, Ma Dolores
AU - Martínez, Mónica Sierra
AU - Arenas Aranda, Diego J.
N1 - Funding Information:
The authors are grateful to Maggie Brunner, and Veronica Vratny, for technical language assessment in English. E C-Q is a COFAA Fellow. Grant information: This work was supported by grants 34451-M and Salud-2003-C01-074 from the Mexican Council of Science and Technology (CONACyT, México), and grants from the Medical Research Council of Mexican Social Security Institute (IMSS) (FOFOI FP-0038/1248, FP-0038/1247, and FP-0038/763 México).
PY - 2006/10/15
Y1 - 2006/10/15
N2 - Breast cancer is the second-leading cause of death among Mexican women >35 years of age. At the molecular level, changes in many genetic pathways have been reported to be associated with this neoplasm. To analyze these changes, we determined gene expression profiles and chromosomal structural alterations in tumors from Mexican women. We obtained mRNA to identify expression profiles with microarray technology, and DNA to determine amplifications and deletions, in 10 fresh sporadic breast tumor biopsies without treatment, as well as in 10 nonaffected breast tissues. Expression profiles were compared with genetic changes observed by comparative genomic hybridization (CGH). We compared the expression profiles against the structural alterations from the studied genes by means of microarrays; at least 17 of these genes correlated with DNA copy number alterations. We found that the following genes were overexpressed: LAMC1, PCTK3, CCNC, CCND1, FGF3, PCTK2, L1CAM, BGN, and PLXNB3 (alias PLEXR). Underexpressed genes included CASP9, FGR, TP73, HSPG2, and ERCC1; genes turned off included FRAP1, EPHA2 (previously ECK), IL12A, E2F5, TNFRSF10B, TNFRSF10A, EFNB3, and BCL2. The results will allow us, in the near future, to outline genes that could serve as diagnostic, prognostic, or target therapy markers for the Mexican population.
AB - Breast cancer is the second-leading cause of death among Mexican women >35 years of age. At the molecular level, changes in many genetic pathways have been reported to be associated with this neoplasm. To analyze these changes, we determined gene expression profiles and chromosomal structural alterations in tumors from Mexican women. We obtained mRNA to identify expression profiles with microarray technology, and DNA to determine amplifications and deletions, in 10 fresh sporadic breast tumor biopsies without treatment, as well as in 10 nonaffected breast tissues. Expression profiles were compared with genetic changes observed by comparative genomic hybridization (CGH). We compared the expression profiles against the structural alterations from the studied genes by means of microarrays; at least 17 of these genes correlated with DNA copy number alterations. We found that the following genes were overexpressed: LAMC1, PCTK3, CCNC, CCND1, FGF3, PCTK2, L1CAM, BGN, and PLXNB3 (alias PLEXR). Underexpressed genes included CASP9, FGR, TP73, HSPG2, and ERCC1; genes turned off included FRAP1, EPHA2 (previously ECK), IL12A, E2F5, TNFRSF10B, TNFRSF10A, EFNB3, and BCL2. The results will allow us, in the near future, to outline genes that could serve as diagnostic, prognostic, or target therapy markers for the Mexican population.
UR - http://www.scopus.com/inward/record.url?scp=33749182371&partnerID=8YFLogxK
U2 - 10.1016/j.cancergencyto.2006.06.002
DO - 10.1016/j.cancergencyto.2006.06.002
M3 - Artículo
SN - 0165-4608
VL - 170
SP - 147
EP - 151
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 2
ER -