TY - JOUR
T1 - Genetic diversity of Mexican brook lamprey Lampetra (Tetrapleurodon) geminis (Alvarez del Villar, 1966)
AU - Mejía, Omar
AU - Polaco, Oscar J.
AU - Zúñiga, Gerardo
N1 - Funding Information:
The authors wish to thank Ana F. Guzmán, Ramón Cisneros, and Rubén Cruz for their help in specimen collection. We are indebted to Jane L. Hayes, Fabián Vargas Mendoza, Edmundo Díaz Pardo, Silvia Mille, Ramón Cisneros, Yolanda Salinas, Celia López-González, and Fernando González-Candelas for reviewing the manuscript and making suggestions. This study was funded by the Coordinación General de Posgrado e Investi-gación del IPN (CGPI-IPN 980379 and 20030678). OM was supported by grants from the Instituto Politécnico Nacional between 1998 and 2000, and the Programa Institucional de Formación de In-vestigadores (PIFI-IPN) in 1999.
PY - 2004/11
Y1 - 2004/11
N2 - Lampreys are the only surviving representatives of the oldest known vertebrates. The Mexican lamprey L. geminis (nonparasitic), is particularly interesting, because it is an endemic, biogeographical relict, and a threatened species. RAPD markers were used to describe genetic diversity in L. geminis A total of 77 specimens were collected from five populations, three in the Río Grande de Morelia-Cuitzeo basin and two in the Río Duero-Lerma-Chapala basin, México. Eighty-eight RAPD markers were obtained from eight primers. Genetic diversity within each population was estimated using Shannon's index (S), heterozygosity (H) and gene diversity (h). These estimates revealed significant variation within populations, although a variance homogeneity test (HOMOVA) showed no significant differences among populations or between basins. Nei genetic distance values indicate a low genetic differentiation among populations. Analysis of molecular variance (AMOVA) indicates that most of the genetic diversity occurs within populations (91.4%), but that a statistically significant amount is found among populations (P < 0.001). Principal coordinates and cluster analyses of RAPD phenotypes show that specimens are not grouped by geographical origin. The genetic diversity found within L. geminispopulations may be explained by its breeding system and an overlapping of generations. The scarce genetic differentiation among populations is likely to the low rate of DNA change that characterizes the lamprey group.
AB - Lampreys are the only surviving representatives of the oldest known vertebrates. The Mexican lamprey L. geminis (nonparasitic), is particularly interesting, because it is an endemic, biogeographical relict, and a threatened species. RAPD markers were used to describe genetic diversity in L. geminis A total of 77 specimens were collected from five populations, three in the Río Grande de Morelia-Cuitzeo basin and two in the Río Duero-Lerma-Chapala basin, México. Eighty-eight RAPD markers were obtained from eight primers. Genetic diversity within each population was estimated using Shannon's index (S), heterozygosity (H) and gene diversity (h). These estimates revealed significant variation within populations, although a variance homogeneity test (HOMOVA) showed no significant differences among populations or between basins. Nei genetic distance values indicate a low genetic differentiation among populations. Analysis of molecular variance (AMOVA) indicates that most of the genetic diversity occurs within populations (91.4%), but that a statistically significant amount is found among populations (P < 0.001). Principal coordinates and cluster analyses of RAPD phenotypes show that specimens are not grouped by geographical origin. The genetic diversity found within L. geminispopulations may be explained by its breeding system and an overlapping of generations. The scarce genetic differentiation among populations is likely to the low rate of DNA change that characterizes the lamprey group.
KW - Mexican brook lamprey
KW - RAPD markers
KW - genetic diversity
KW - genetic structure
UR - http://www.scopus.com/inward/record.url?scp=8544225068&partnerID=8YFLogxK
U2 - 10.1007/s10709-004-3127-8
DO - 10.1007/s10709-004-3127-8
M3 - Artículo
SN - 0016-6707
VL - 122
SP - 325
EP - 333
JO - Genetica
JF - Genetica
IS - 3
ER -