TY - JOUR
T1 - Genetic diversity among multidrug-resistant Mycobacterium tuberculosis strains in Mexico
AU - Martinez-Guarneros, Armando
AU - Rastogi, Nalin
AU - Couvin, David
AU - Escobar-Gutierrez, Alejandro
AU - Rossi, Livia Maria Gonçalves
AU - Vazquez-Chacon, Carlos A.
AU - Rivera-Gutierrez, Sandra
AU - Lozano, Daniela
AU - Vergara-Castañeda, Arely
AU - Gonzalez-y-Merchand, Jorge A.
AU - Vaughan, Gilberto
N1 - Funding Information:
David Couvin was awarded a Ph.D. fellowship by the European Social Funds through the Regional Council of Guadeloupe. This work was partially supported by CONACYT- SALUD-2010-C01-139760 and as part of the 7th Framework Program, European Commission, contract No. HEALTH-F3-2008-200999, and by grants CB-2010-156347, CONACYT and SIP20121271, IPN, México. JAG-y-M and SR-G are fellows of COFAA and EDI, IPN, México.
PY - 2013/3
Y1 - 2013/3
N2 - Tuberculosis is an important public health problem in Mexico. However, limited information about the genetic diversity of Mycobacterium tuberculosis strains circulating in the country is available. In this work, 109 multidrug-resistant (MDR) M. tuberculosis isolates collected in 23 different states of Mexico in 2003 were retrospectively characterized by spoligotyping and MIRU-VNTRs. All isolates, except for a single cluster containing two strains (subcluster E1), were split when information from the 12-loci MIRUs and spoligo-pattern was simultaneously analyzed. The discriminative power of 12-loci MIRU-VNTR and spoligotyping, by the Hunter-Gaston index, were 0.9998 and 0.9011, respectively. These findings suggest that almost all cases were epidemiologically unrelated. Instead, the genetic variations observed among these strains are suggestive of emergence of acquired drug-resistance during the course of treatment. The results suggest a high degree of genetic variability and a high frequency of SIT53 (T1 family) spoligotype among the MDR M. tuberculosis isolates included in the study.
AB - Tuberculosis is an important public health problem in Mexico. However, limited information about the genetic diversity of Mycobacterium tuberculosis strains circulating in the country is available. In this work, 109 multidrug-resistant (MDR) M. tuberculosis isolates collected in 23 different states of Mexico in 2003 were retrospectively characterized by spoligotyping and MIRU-VNTRs. All isolates, except for a single cluster containing two strains (subcluster E1), were split when information from the 12-loci MIRUs and spoligo-pattern was simultaneously analyzed. The discriminative power of 12-loci MIRU-VNTR and spoligotyping, by the Hunter-Gaston index, were 0.9998 and 0.9011, respectively. These findings suggest that almost all cases were epidemiologically unrelated. Instead, the genetic variations observed among these strains are suggestive of emergence of acquired drug-resistance during the course of treatment. The results suggest a high degree of genetic variability and a high frequency of SIT53 (T1 family) spoligotype among the MDR M. tuberculosis isolates included in the study.
KW - MDR-TB
KW - MIRU-VNTR
KW - Mycobacterium tuberculosis
KW - Spoligotyping
UR - http://www.scopus.com/inward/record.url?scp=84873645095&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2012.12.024
DO - 10.1016/j.meegid.2012.12.024
M3 - Artículo
SN - 1567-1348
VL - 14
SP - 434
EP - 443
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
IS - 1
ER -