TY - JOUR
T1 - Gene-sensor on the Basis of Magnetic Immobilization and Bioimpedance Measurements
T2 - 3rd Latin-American Conference on Bioimpedance, CLABIO 2018
AU - Laguna, D. U.
AU - Sanchez, V.
AU - Ames, G.
AU - González, C. A.
N1 - Publisher Copyright:
© 2019 Published under licence by IOP Publishing Ltd.
PY - 2019/9/10
Y1 - 2019/9/10
N2 - Detection of molecular markers or specific DNA sequences (genes) represents the future in genomic medicine. Gene detection requires expensive equipment and specialized technicians, thus biosensors development that allows such detection quickly, economically and simply is fundamental in the first level of health care. In this study the development and evaluation of a genosensor based on magnetic anchoring of DNA sequences and electrical bioimpedance spectroscopy measurements is presented. As a first proof of concept, bioimpedance measurements were developed in PCR product anchored by magnetic nanoparticles and two conditions were compared; PCR product and absence of amplicons. The experimental evaluation indicates technical feasibility of developing a biosensor of molecular markers or specific genes trough electric bioimpedance measurements assisted with magnetic nanoparticles. The observations show that the DNA biosensor proposal offers the possibility of detecting a PCR product and differentiating it from the absence of amplicons.
AB - Detection of molecular markers or specific DNA sequences (genes) represents the future in genomic medicine. Gene detection requires expensive equipment and specialized technicians, thus biosensors development that allows such detection quickly, economically and simply is fundamental in the first level of health care. In this study the development and evaluation of a genosensor based on magnetic anchoring of DNA sequences and electrical bioimpedance spectroscopy measurements is presented. As a first proof of concept, bioimpedance measurements were developed in PCR product anchored by magnetic nanoparticles and two conditions were compared; PCR product and absence of amplicons. The experimental evaluation indicates technical feasibility of developing a biosensor of molecular markers or specific genes trough electric bioimpedance measurements assisted with magnetic nanoparticles. The observations show that the DNA biosensor proposal offers the possibility of detecting a PCR product and differentiating it from the absence of amplicons.
UR - http://www.scopus.com/inward/record.url?scp=85073417408&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1272/1/012020
DO - 10.1088/1742-6596/1272/1/012020
M3 - Artículo de la conferencia
AN - SCOPUS:85073417408
SN - 1742-6588
VL - 1272
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012020
Y2 - 3 October 2018 through 5 October 2018
ER -