TY - JOUR
T1 - Gene-sensor on the basis of bioimpedance measurements assisted with nanotechnology
T2 - 3rd Latin-American Conference on Bioimpedance, CLABIO 2018
AU - Ames, G.
AU - Laguna, D. U.
AU - Sánchez, V.
AU - Sacristán, E.
AU - González, C. A.
N1 - Publisher Copyright:
© 2019 Published under licence by IOP Publishing Ltd.
PY - 2019/9/10
Y1 - 2019/9/10
N2 - In this study, we propose basic instrumentation for a new gene-sensor based on Polymerase Chain Reaction (PCR) product and its multifrequency bioimpedance relative measurements assisted with nanotechnology. The instrumentation proposal is split into four basic blocks: A) Control.-The fundament is a microcontroller (C) for wide bandwidth current excitation, as well as data acquisition and registration. B) Digital Synthesizer.-A wide bandwidth digital synthesizer will provide a sinusoidal excitation current. C) Bioimpedance measurement.-this section will detect relative multifrequency bioimpedance by comparison of a reference signal with the signal of the Analyte Under Test (AUT). Comparison is on the basis of magnitude ratio and phase shift. D) Electrical-Ionic Interphase.-This is based on gold electrodes in contact with the AUT for current injection and relative bioimpedance measurements. Deoxyribonucleic Acid (DNA) fragments as PCR products will be functionalized by magnetic nanoparticles-DNA biding and its magnetic DNA insulation. The specific electronic components for every section are out of the range of this study, and the general instrumentation structure proposed is intended to show tentative low cost technology for gene detection and obtain academic discussion and feedback.
AB - In this study, we propose basic instrumentation for a new gene-sensor based on Polymerase Chain Reaction (PCR) product and its multifrequency bioimpedance relative measurements assisted with nanotechnology. The instrumentation proposal is split into four basic blocks: A) Control.-The fundament is a microcontroller (C) for wide bandwidth current excitation, as well as data acquisition and registration. B) Digital Synthesizer.-A wide bandwidth digital synthesizer will provide a sinusoidal excitation current. C) Bioimpedance measurement.-this section will detect relative multifrequency bioimpedance by comparison of a reference signal with the signal of the Analyte Under Test (AUT). Comparison is on the basis of magnitude ratio and phase shift. D) Electrical-Ionic Interphase.-This is based on gold electrodes in contact with the AUT for current injection and relative bioimpedance measurements. Deoxyribonucleic Acid (DNA) fragments as PCR products will be functionalized by magnetic nanoparticles-DNA biding and its magnetic DNA insulation. The specific electronic components for every section are out of the range of this study, and the general instrumentation structure proposed is intended to show tentative low cost technology for gene detection and obtain academic discussion and feedback.
UR - http://www.scopus.com/inward/record.url?scp=85073423241&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1272/1/012023
DO - 10.1088/1742-6596/1272/1/012023
M3 - Artículo de la conferencia
AN - SCOPUS:85073423241
SN - 1742-6588
VL - 1272
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012023
Y2 - 3 October 2018 through 5 October 2018
ER -