TY - JOUR
T1 - Gastroprotective Properties of Nanoemulsion of Ligusticum porteri Volatile Oil in Rats
AU - Pineda-Peña, Elizabeth Arlen
AU - Vega-Aguilar, Itzel Karina
AU - Chávez-Piña, Aracely Evangelina
AU - Velázquez-Moyado, Josué Arturo
AU - Tavares-Carvalho, José Carlos
AU - Navarrete, Andrés
N1 - Publisher Copyright:
© 2020, Sociedade Brasileira de Farmacognosia.
PY - 2020/4
Y1 - 2020/4
N2 - The roots of Ligusticum porteri J.M. Coult. & Rose, Apiaceae, are used in Mexican traditional medicine for the treatment of gastrointestinal disorders. The dimeric phthalide (Z´)-diligustilide found in their organic extracts is associated with the gastroprotective properties. In this study, the gastroprotective effect of nanoemulsion of the volatile oil from the roots of L. porteri (at doses of 1, 3, 10, and 30 mg/kg) in the indomethacin-induced gastric damage model was tested, and nitric oxide (NO) and hydrogen sulfide (H2S) gastric levels were determinate. The volatile oil obtained by steam distillation (0.63% yield) contained 37 constituents, as assessed by GC-MS analysis, where the significant compounds were 4-phenyl-3-buten-2-ol (20.54%) and (Z)-3-butylidenephthalide (20.54%) that correspond to phthalide monomers. These results demonstrate that nanoemulsion of the volatile oil from L. porteri (at 10 and 30 mg/kg) shows a significant gastroprotective effect (72 and 92% respectively) against indomethacin-induced gastric damage, which is not different from the gastroprotective effect of omeprazole (69%) (p < 0.05). Furthermore, these doses of the nanoemulsion maintain the basal H2S gastric levels (209.70 ± 11.50 and 222.0 ± 5.30 nmol/g tissue) even in the presence of indomethacin. From these findings, we concluded that L. porteri volatile oil in nanoemulsion preparation might facilitate the gastroprotective action and it could serve as a future therapeutic approach for NSAID-related gastropathy.
AB - The roots of Ligusticum porteri J.M. Coult. & Rose, Apiaceae, are used in Mexican traditional medicine for the treatment of gastrointestinal disorders. The dimeric phthalide (Z´)-diligustilide found in their organic extracts is associated with the gastroprotective properties. In this study, the gastroprotective effect of nanoemulsion of the volatile oil from the roots of L. porteri (at doses of 1, 3, 10, and 30 mg/kg) in the indomethacin-induced gastric damage model was tested, and nitric oxide (NO) and hydrogen sulfide (H2S) gastric levels were determinate. The volatile oil obtained by steam distillation (0.63% yield) contained 37 constituents, as assessed by GC-MS analysis, where the significant compounds were 4-phenyl-3-buten-2-ol (20.54%) and (Z)-3-butylidenephthalide (20.54%) that correspond to phthalide monomers. These results demonstrate that nanoemulsion of the volatile oil from L. porteri (at 10 and 30 mg/kg) shows a significant gastroprotective effect (72 and 92% respectively) against indomethacin-induced gastric damage, which is not different from the gastroprotective effect of omeprazole (69%) (p < 0.05). Furthermore, these doses of the nanoemulsion maintain the basal H2S gastric levels (209.70 ± 11.50 and 222.0 ± 5.30 nmol/g tissue) even in the presence of indomethacin. From these findings, we concluded that L. porteri volatile oil in nanoemulsion preparation might facilitate the gastroprotective action and it could serve as a future therapeutic approach for NSAID-related gastropathy.
KW - Gastroprotection
KW - Hydrogen sulfide
KW - Indomethacin-induced gastric ulcer
KW - Nanoemulsion
KW - Volatile oil
UR - http://www.scopus.com/inward/record.url?scp=85133709748&partnerID=8YFLogxK
U2 - 10.1007/s43450-020-00039-z
DO - 10.1007/s43450-020-00039-z
M3 - Artículo
AN - SCOPUS:85133709748
SN - 0102-695X
VL - 30
SP - 261
EP - 271
JO - Revista Brasileira de Farmacognosia
JF - Revista Brasileira de Farmacognosia
IS - 2
ER -