TY - JOUR
T1 - Garlic ameliorates gentamicin nephrotoxicity
T2 - Relation to antioxidant enzymes
AU - Pedraza-Chaverrí, José
AU - Maldonado, Perla D.
AU - Medina-Campos, Omar N.
AU - Olivares-Corichi, Ivonne M.
AU - Granados-Silvestre, María De Los Ángeles
AU - Hernández-Pando, Rogelio
AU - Ibarra-Rubio, María E.
N1 - Funding Information:
This work was supported by DGAPA (IN203797) and Facultad de Quı́mica (PAIP 6190-03) from the National Autonomous University of Mexico (UNAM) and CONACYT (25441-M).We thank QFB Romelia Velasco and QFB Tere Murguı́a of Instituto Nacional de Pediatrı́a for the measurement of renal cortical GM concentration.
PY - 2000
Y1 - 2000
N2 - Reactive oxygen species are involved in gentamicin (GM) nephrotoxicity, and garlic is effective in preventing or ameliorating oxidative stress. Therefore, the effect of garlic on GM nephrotoxicity was investigated in this work. Four groups of rats were studied: (i) fed normal diet (CT), (ii) treated with GM (GM), (iii) fed 2% garlic diet (GA), and (iv) treated with GM and 2% garlic diet (GM + GA). Rats were placed in metabolic cages and GM nephrotoxicity was induced by injections of GM (75 mg/kg every 12 h) for 6 d. Lipoperoxidation and enzyme determinations were made in renal cortex on day 7. GM nephrotoxicity was made evident on day 7 by (i) tubular histological damage, (ii) enhanced BUN and urinary excretion of N-acetyl-β-D-glucosaminidase, and (iii) decreased creatinine clearance. These alterations were prevented or ameliorated in GM + GA group. The rise in lipoperoxidation and the decrease in Mn-SOD and glutathione peroxidase (GPx) activities observed in the GM group, were prevented in the GM + GA group. Cu,Zn-SOD activity and Mn-SOD and Cu,Zn-SOD content did not change. CAT activity and content decreased in the GM, GA, and GM + GA groups. CAT mRNA levels decreased in the GM group. The protective effect of garlic is associated with the prevention of the decrease of Mn-SOD and GPx activities and with the rise of lipoperoxidation in renal cortex. Copyright (C) 2000 Elsevier Science Inc.
AB - Reactive oxygen species are involved in gentamicin (GM) nephrotoxicity, and garlic is effective in preventing or ameliorating oxidative stress. Therefore, the effect of garlic on GM nephrotoxicity was investigated in this work. Four groups of rats were studied: (i) fed normal diet (CT), (ii) treated with GM (GM), (iii) fed 2% garlic diet (GA), and (iv) treated with GM and 2% garlic diet (GM + GA). Rats were placed in metabolic cages and GM nephrotoxicity was induced by injections of GM (75 mg/kg every 12 h) for 6 d. Lipoperoxidation and enzyme determinations were made in renal cortex on day 7. GM nephrotoxicity was made evident on day 7 by (i) tubular histological damage, (ii) enhanced BUN and urinary excretion of N-acetyl-β-D-glucosaminidase, and (iii) decreased creatinine clearance. These alterations were prevented or ameliorated in GM + GA group. The rise in lipoperoxidation and the decrease in Mn-SOD and glutathione peroxidase (GPx) activities observed in the GM group, were prevented in the GM + GA group. Cu,Zn-SOD activity and Mn-SOD and Cu,Zn-SOD content did not change. CAT activity and content decreased in the GM, GA, and GM + GA groups. CAT mRNA levels decreased in the GM group. The protective effect of garlic is associated with the prevention of the decrease of Mn-SOD and GPx activities and with the rise of lipoperoxidation in renal cortex. Copyright (C) 2000 Elsevier Science Inc.
KW - Catalase
KW - Cu
KW - Free radicals
KW - Garlic
KW - Gentamicin
KW - Glutathione peroxidase
KW - Lipoperoxidation
KW - Mn-SOD
KW - Nephrotoxicity
KW - Zn-SOD
UR - http://www.scopus.com/inward/record.url?scp=0033804999&partnerID=8YFLogxK
U2 - 10.1016/S0891-5849(00)00354-3
DO - 10.1016/S0891-5849(00)00354-3
M3 - Artículo
C2 - 11033412
AN - SCOPUS:0033804999
SN - 0891-5849
VL - 29
SP - 602
EP - 611
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 7
ER -