TY - JOUR
T1 - Frequency of Toxocara canis antibodies in Mexican paediatric patients with epilepsy
AU - Caballero-García, M. D.L.
AU - Simón-Salvador, J.
AU - Hernández-Aguilar, J. C.
AU - Reyes-Lopez, A.
AU - Nogueda-Torres, B.
AU - Jiménez-Cardoso, E.
N1 - Publisher Copyright:
Copyright © Cambridge University Press 2019Â.
PY - 2020
Y1 - 2020
N2 - The relationship between epilepsy and the presence of visceral larva migrans caused by Toxocara canis in Mexican children remains uncertain; however, this relationship needs to be elucidated because these parasite larvae can invade the human central nervous system. Accordingly, this study aimed to determine the frequency and specificity of anti-T. Canis antibodies in the sera of children with epilepsy to determine the relationship between this parasite and epilepsy. The sera samples of 214 children were examined: 111 children diagnosed with epilepsy and 103 clinically healthy children without neurological disorders. In the sera of each group, the presence and specificity of anti-T. Canis and anti-Ascaris lumbricoides antibodies, as well as the cross-reactivity between them, were assessed using enzyme-linked immunosorbent assay and Western blotting analysis. Among the children with epilepsy, 25.2% exhibited seropositivity to T. Canis. Cross-reactivity against the A. Lumbricoides antigen was present in 46.8% of the children with epilepsy, whereas 11.7% of the children with epilepsy and anti-T. Canis antibodies did not exhibit cross-reactivity against this antigen. The Western blotting analysis of the sera from the children with epilepsy demonstrated the presence of T. Canis proteins, with molecular weights of 24, 35, 55, 70, 120 and 210 kDa, and A lumbricoides proteins with molecular weights of 70, 80 and 110 kDa. Our results revealed the presence of anti-T. Canis antibodies in the children with epilepsy; furthermore, cross-reactivity tests with A. Lumbricoides showed the importance of the presence of anti-T. Canis antibodies in revealing the relationship between this parasite and epilepsy in children.
AB - The relationship between epilepsy and the presence of visceral larva migrans caused by Toxocara canis in Mexican children remains uncertain; however, this relationship needs to be elucidated because these parasite larvae can invade the human central nervous system. Accordingly, this study aimed to determine the frequency and specificity of anti-T. Canis antibodies in the sera of children with epilepsy to determine the relationship between this parasite and epilepsy. The sera samples of 214 children were examined: 111 children diagnosed with epilepsy and 103 clinically healthy children without neurological disorders. In the sera of each group, the presence and specificity of anti-T. Canis and anti-Ascaris lumbricoides antibodies, as well as the cross-reactivity between them, were assessed using enzyme-linked immunosorbent assay and Western blotting analysis. Among the children with epilepsy, 25.2% exhibited seropositivity to T. Canis. Cross-reactivity against the A. Lumbricoides antigen was present in 46.8% of the children with epilepsy, whereas 11.7% of the children with epilepsy and anti-T. Canis antibodies did not exhibit cross-reactivity against this antigen. The Western blotting analysis of the sera from the children with epilepsy demonstrated the presence of T. Canis proteins, with molecular weights of 24, 35, 55, 70, 120 and 210 kDa, and A lumbricoides proteins with molecular weights of 70, 80 and 110 kDa. Our results revealed the presence of anti-T. Canis antibodies in the children with epilepsy; furthermore, cross-reactivity tests with A. Lumbricoides showed the importance of the presence of anti-T. Canis antibodies in revealing the relationship between this parasite and epilepsy in children.
KW - Toxocara canis
KW - antibodies
KW - antigens
KW - epilepsy
KW - serology
UR - http://www.scopus.com/inward/record.url?scp=85072658711&partnerID=8YFLogxK
U2 - 10.1017/S0022149X1900083X
DO - 10.1017/S0022149X1900083X
M3 - Artículo
C2 - 31544721
SN - 0022-149X
VL - 94
JO - Journal of Helminthology
JF - Journal of Helminthology
M1 - e89
ER -