TY - JOUR
T1 - Fractionation and electrophoretic patterns of seed protein of Opuntia genus. A preliminary survey as a tool for accession differentiation and taxonomy
AU - Samah, Samir
AU - Ventura-Zapata, Elsa
AU - Valadez-Moctezuma, Ernestina
N1 - Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - At present, little is known about Opuntia seed proteins and their contribution to the characterization and taxonomy of genotypes belong to this genus. The variation among 102 accessions of Mexican Opuntia was studied using electrophoretic patterns by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of their seed total proteins (STPs) and seed storage proteins (SSPs). Albumins and globulins were the most abundant protein fractions, with contents varied from 2.6 to 11.9mg/mL and 2.6-9.5mg/mL, respectively. These were followed by glutelins (2.3-8.5mg/mL) and prolamins as the lowest (1.1-7.9mg/mL) of the four protein factions. On the other hand, STPs content varied between 1.13 and 7.12mg/mL. Regardless of variations in protein content estimated in seeds, the electrophoretic patterns of STPs and SSPs, as outcome of their SDS-PAGE, were not so variable. Furthermore, the individual analysis of each STPs or the SSPs analyses, separately, were not enough to discriminate all accessions, since it was necessary to combine the data resulting from all protein profiles together to differentiate all Opuntia genotypes. The UPGMA analysis indicated that there is no separation between accessions of species of the prickly pear (sweet fruits) and ". xoconostle" (acidic fruits), even though the latter were grouped together. Based on biochemical markers analyzed herein, the need for revision of taxonomic assignment of genotypes belonging to the genus Opuntia is suggested.
AB - At present, little is known about Opuntia seed proteins and their contribution to the characterization and taxonomy of genotypes belong to this genus. The variation among 102 accessions of Mexican Opuntia was studied using electrophoretic patterns by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of their seed total proteins (STPs) and seed storage proteins (SSPs). Albumins and globulins were the most abundant protein fractions, with contents varied from 2.6 to 11.9mg/mL and 2.6-9.5mg/mL, respectively. These were followed by glutelins (2.3-8.5mg/mL) and prolamins as the lowest (1.1-7.9mg/mL) of the four protein factions. On the other hand, STPs content varied between 1.13 and 7.12mg/mL. Regardless of variations in protein content estimated in seeds, the electrophoretic patterns of STPs and SSPs, as outcome of their SDS-PAGE, were not so variable. Furthermore, the individual analysis of each STPs or the SSPs analyses, separately, were not enough to discriminate all accessions, since it was necessary to combine the data resulting from all protein profiles together to differentiate all Opuntia genotypes. The UPGMA analysis indicated that there is no separation between accessions of species of the prickly pear (sweet fruits) and ". xoconostle" (acidic fruits), even though the latter were grouped together. Based on biochemical markers analyzed herein, the need for revision of taxonomic assignment of genotypes belonging to the genus Opuntia is suggested.
KW - Biochemical markers
KW - Characterization
KW - Nopal
KW - SDS-PAGE
UR - http://www.scopus.com/inward/record.url?scp=84920148668&partnerID=8YFLogxK
U2 - 10.1016/j.bse.2014.12.005
DO - 10.1016/j.bse.2014.12.005
M3 - Artículo
SN - 0305-1978
VL - 58
SP - 187
EP - 194
JO - Biochemical Systematics and Ecology
JF - Biochemical Systematics and Ecology
ER -