TY - JOUR
T1 - Flagella, type i fimbriae and curli of uropathogenic escherichia coli promote the release of proinflammatory cytokines in a coculture system
AU - Vega-Hernández, Rubí
AU - Ochoa, Sara A.
AU - Valle-Rios, Ricardo
AU - Jaimes-Ortega, Gustavo A.
AU - Arellano-Galindo, José
AU - Aparicio-Ozores, Gerardo
AU - Ibarra, José Antonio
AU - Hernández-Castro, Rigoberto
AU - Cruz-Córdova, Ariadnna
AU - Xicohtencatl-Cortes, Juan
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/11
Y1 - 2021/11
N2 - al n l-Abstract: Background. Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic Escherichia coli (UPEC) is one of the main etiological agents. Flagella, type I fimbriae, and curli promote the ability of these bacteria to successfully colonize its host. Aim. This study aimed to determine whether flagella-, type I fimbriae-, and curli-expressing UPEC induces the release of proinflammatory cytokines in an established coculture system. Methods. The fliC, fimH, and csgA genes by UPEC strain were disrupted by allelic replacement. Flagella, type I fimbriae, and curli were visualized by transmission electron microscopy (TEM). HTB-5 (upper chamber) and HMC-1 (lower chamber) cells cocultured in Transwell® plates were infected with these UPEC strains and purified proteins. There was adherence to HTB-5 cells treated with different UPEC strains and they were quantified as colony-forming units (CFU)/mL. Results. High concentrations of IL-6 and IL-8 were induced by the FimH and FliC proteins; however, these cytokines were detected in low concentrations in presence of CsgA. Compared with UPEC CFT073, CFT073∆fimH, CFT073∆fimH∆fliC, and CFT073∆csgA∆fimH strains significantly reduced the adherence to HTB-5 cells. Conclusion. The FimH and FliC proteins are involved in IL-6 and IL-8 release in a coculture model of HTB-5 and HMC-1 cells.
AB - al n l-Abstract: Background. Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic Escherichia coli (UPEC) is one of the main etiological agents. Flagella, type I fimbriae, and curli promote the ability of these bacteria to successfully colonize its host. Aim. This study aimed to determine whether flagella-, type I fimbriae-, and curli-expressing UPEC induces the release of proinflammatory cytokines in an established coculture system. Methods. The fliC, fimH, and csgA genes by UPEC strain were disrupted by allelic replacement. Flagella, type I fimbriae, and curli were visualized by transmission electron microscopy (TEM). HTB-5 (upper chamber) and HMC-1 (lower chamber) cells cocultured in Transwell® plates were infected with these UPEC strains and purified proteins. There was adherence to HTB-5 cells treated with different UPEC strains and they were quantified as colony-forming units (CFU)/mL. Results. High concentrations of IL-6 and IL-8 were induced by the FimH and FliC proteins; however, these cytokines were detected in low concentrations in presence of CsgA. Compared with UPEC CFT073, CFT073∆fimH, CFT073∆fimH∆fliC, and CFT073∆csgA∆fimH strains significantly reduced the adherence to HTB-5 cells. Conclusion. The FimH and FliC proteins are involved in IL-6 and IL-8 release in a coculture model of HTB-5 and HMC-1 cells.
KW - Adherence
KW - Coculture
KW - Cytokines
KW - Fimbriae
KW - UPEC
UR - http://www.scopus.com/inward/record.url?scp=85117950491&partnerID=8YFLogxK
U2 - 10.3390/microorganisms9112233
DO - 10.3390/microorganisms9112233
M3 - Artículo
C2 - 34835359
AN - SCOPUS:85117950491
SN - 2076-2607
VL - 9
JO - Microorganisms
JF - Microorganisms
IS - 11
M1 - 2233
ER -