TY - JOUR
T1 - First insights into the genetic diversity of Mycobacterium tuberculosis isolates from HIV-infected Mexican patients and mutations causing multidrug resistance
AU - Lopez-Alvarez, Rocio
AU - Badillo-Lopez, Claudia
AU - Cerna-Cortes, Jorge F.
AU - Castillo-Ramirez, Ivan
AU - Rivera-Gutierrez, Sandra
AU - Helguera-Repetto, Addy C.
AU - Aguilar, Diana
AU - Hernandez-Pando, Rogelio
AU - Samper, Sofia
AU - Gonzalez-Y-Merchand, Jorge A.
N1 - Funding Information:
We thank Dr Mildred Foster, PhD, for helpful review of the manuscript and Alberto Cebollada (Zaragoza, Spain) for his help with spoligotyping analysis. This work was supported by CONACyT, Mexico, grant 2006-P60954 (JFC-C), Network 07RT0311 Program CYTED Spain (SS and JAG-y-M), and European Community, grant No. HEALTH-F3-2008-200999. It was also in part supported by IPN, SIP, grants No. 20090084 and 20091259. JFC-C, SR-G and JAG-y-M are fellows of COFAA and EDI, IPN, Mexico.
PY - 2010
Y1 - 2010
N2 - Background. The prevalence of infections with Mycobacterium tuberculosis (MTb) and nontuberculous mycobacteria (NTM) species in HIV-infected patients in Mexico is unknown. The aims of this study were to determine the frequency of MTb and NTM species in HIV-infected patients from Mexico City, to evaluate the genotypic diversity of the Mycobacterium tuberculosis complex strains, to determine their drug resistance profiles by colorimetric microplate Alamar Blue assay (MABA), and finally, to detect mutations present in katG, rpoB and inhA genes, resulting in isoniazid (INH) and rifampin (RIF) resistance. Results. Of the 67 mycobacterial strains isolated, 48 were identified as MTb, 9 as M. bovis, 9 as M. avium and 1 as M. intracellulare. IS6110-RFLP of 48 MTb strains showed 27 profiles. Spoligotyping of the 48 MTb strains yielded 21 patterns, and 9 M. bovis strains produced 7 patterns. Eleven new spoligotypes patterns were found. A total of 40 patterns were produced from the 48 MTb strains when MIRU-VNTR was performed. Nineteen (39.6%) MTb strains were resistant to one or more drugs. One (2.1%) multidrug-resistant (MDR) strain was identified. A novel mutation was identified in a RIF-resistant strain, GAG TCG (Glu Ser) at codon 469 of rpoB gene. Conclusions. This is the first molecular analysis of mycobacteria isolated from HIV-infected patients in Mexico, which describe the prevalence of different mycobacterial species in this population. A high genetic diversity of MTb strains was identified. New spoligotypes and MIRU-VNTR patterns as well as a novel mutation associated to RIF-resistance were found. This information will facilitate the tracking of different mycobacterial species in HIV-infected individuals, and monitoring the spread of these microorganisms, leading to more appropriate measures for tuberculosis control.
AB - Background. The prevalence of infections with Mycobacterium tuberculosis (MTb) and nontuberculous mycobacteria (NTM) species in HIV-infected patients in Mexico is unknown. The aims of this study were to determine the frequency of MTb and NTM species in HIV-infected patients from Mexico City, to evaluate the genotypic diversity of the Mycobacterium tuberculosis complex strains, to determine their drug resistance profiles by colorimetric microplate Alamar Blue assay (MABA), and finally, to detect mutations present in katG, rpoB and inhA genes, resulting in isoniazid (INH) and rifampin (RIF) resistance. Results. Of the 67 mycobacterial strains isolated, 48 were identified as MTb, 9 as M. bovis, 9 as M. avium and 1 as M. intracellulare. IS6110-RFLP of 48 MTb strains showed 27 profiles. Spoligotyping of the 48 MTb strains yielded 21 patterns, and 9 M. bovis strains produced 7 patterns. Eleven new spoligotypes patterns were found. A total of 40 patterns were produced from the 48 MTb strains when MIRU-VNTR was performed. Nineteen (39.6%) MTb strains were resistant to one or more drugs. One (2.1%) multidrug-resistant (MDR) strain was identified. A novel mutation was identified in a RIF-resistant strain, GAG TCG (Glu Ser) at codon 469 of rpoB gene. Conclusions. This is the first molecular analysis of mycobacteria isolated from HIV-infected patients in Mexico, which describe the prevalence of different mycobacterial species in this population. A high genetic diversity of MTb strains was identified. New spoligotypes and MIRU-VNTR patterns as well as a novel mutation associated to RIF-resistance were found. This information will facilitate the tracking of different mycobacterial species in HIV-infected individuals, and monitoring the spread of these microorganisms, leading to more appropriate measures for tuberculosis control.
UR - http://www.scopus.com/inward/record.url?scp=77950193660&partnerID=8YFLogxK
U2 - 10.1186/1471-2180-10-82
DO - 10.1186/1471-2180-10-82
M3 - Artículo
C2 - 20236539
SN - 1471-2180
VL - 10
JO - BMC Microbiology
JF - BMC Microbiology
M1 - 82
ER -