TY - JOUR
T1 - Expression of the Bacterial Hemoglobin Gene from Vitreoscilla stercoraria Increases Rifamycin B Production in Amycolatopsis mediterranei
AU - Priscila, Guerra
AU - Fernández, Francisco J.
AU - Absalón, Angel E.
AU - Suarez, Ma Del Rocío
AU - Sainoz, Mara
AU - Barrios-González, Javier
AU - Mejía, Armando
N1 - Funding Information:
The authors wish to thank the Consejo Nacional de Ciencia y Tecnología (CONACyT, México) for financial support (grant SEP-2003-C02-44911).
PY - 2008/11
Y1 - 2008/11
N2 - It is well known that the culture for rifamycin B production by Amycolatopsis mediterranei requires high levels of dissolved oxygen, particularly in industrial processes. In this study, we report the construction of a vector for the expression of the bacterial hemoglobin gene (vhb) from Vitreoscilla stercoraria in a rifamycin B-overproducing strain of A. mediterranei. The effect was evaluated in the presence and absence of barbital. The vhb gene was cloned under the control of the PermE promoter, the Amycolatopsis lactamdurans plasmid pULVK2 origin of replication, the kanamycin-resistant gene (Km), the erythromycin-resistant gene (ermE) for selection, and ColE1. Industrial fermentation conditions were simulated in shake-flask cultures. Under low aeration, the transformed A. mediterranei strain with the vhb gene showed a 13.9% higher production of rifamycin B in a culture with barbital compared with the parental strain, and 29.5% higher production under the same conditions without barbital.
AB - It is well known that the culture for rifamycin B production by Amycolatopsis mediterranei requires high levels of dissolved oxygen, particularly in industrial processes. In this study, we report the construction of a vector for the expression of the bacterial hemoglobin gene (vhb) from Vitreoscilla stercoraria in a rifamycin B-overproducing strain of A. mediterranei. The effect was evaluated in the presence and absence of barbital. The vhb gene was cloned under the control of the PermE promoter, the Amycolatopsis lactamdurans plasmid pULVK2 origin of replication, the kanamycin-resistant gene (Km), the erythromycin-resistant gene (ermE) for selection, and ColE1. Industrial fermentation conditions were simulated in shake-flask cultures. Under low aeration, the transformed A. mediterranei strain with the vhb gene showed a 13.9% higher production of rifamycin B in a culture with barbital compared with the parental strain, and 29.5% higher production under the same conditions without barbital.
KW - Actinomycetes
KW - Amycolatopsis
KW - Vitreoscilla
KW - bacterial hemoglobin
KW - barbital
KW - rifamycin
UR - http://www.scopus.com/inward/record.url?scp=57849163561&partnerID=8YFLogxK
U2 - 10.1263/jbb.106.493
DO - 10.1263/jbb.106.493
M3 - Artículo
SN - 1389-1723
VL - 106
SP - 493
EP - 497
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 5
ER -