TY - JOUR
T1 - Expression of Pregnancy Specific β-1 Glycoprotein 1 in Cervical Cancer Cells
AU - Rodríguez-Esquivel, Miriam
AU - Romero-Morelos, Pablo
AU - Taniguchi-Ponciano, Keiko
AU - Mendoza-Rodríguez, Mónica
AU - Marrero-Rodríguez, Daniel
AU - Bandera-Delgado, Arfy
AU - Huerta-Padilla, Victor
AU - Serna-Reyna, Luis
AU - Gómez-Gutiérrez, Guillermo
AU - Gómez-Virgilio, Laura
AU - Bandala, Cindy
AU - López-Romero, Ricardo
AU - Garrido-Guerrero, Efraín
AU - Chanona-Pérez, Jorge
AU - Salcedo, Mauricio
N1 - Publisher Copyright:
© 2020 IMSS
PY - 2020/8
Y1 - 2020/8
N2 - Background: Cervical Cancer (CC) is a worldwide public health concern associated with genetic alterations, among these the gain of the 19q chromosome harboring the Pregnancy Specific Glycoproteins (PSG) gene family. These proteins play a critical role in pregnancy, with participation in immunotolerance, angiogenesis, and invasion processes, which are also observed in carcinogenesis. The aim of this study was to determine the molecular alterations of PSG1 and its relationship with CC. Methods: PSG1 Copy Number Variation (CNV) was evaluated in 31 CC and eight normal cervical tissues by qPCR. PSG1 expression was correlated with HPV detection and IL-10 and TGF-β expression in CC samples. Finally, PSG1 protein expression was evaluated by immunofluorescence in CC cell lines, by immunohistochemistry in a tissue microarray, and by immunoblotting in the sera of women with normal cervix, pre-invasive lesions, and CC. Results: PSG1 showed a gain of 25.6% in CNV and gene expression in CC. There was a lack of PSG1 expression in normal cervical epithelium and positive immunostaining in 57% of CC tissues, while all CC cell lines expressed PSG1. Finally, PSG1 was immunodetected in 90% of pre-invasive lesions and in all CC serum samples, but not in healthy women. PSG1 expression correlates with the expression of IL-10 and TGF-β in CC tissues, but not with the presence of HPV. Conclusion: These data show evidence of the differential expression of PSG1 in CC that could explain its participation in tumor-biology and immunotolerance mechanisms. Further, its immunodetection could provide early detection of this cancer.
AB - Background: Cervical Cancer (CC) is a worldwide public health concern associated with genetic alterations, among these the gain of the 19q chromosome harboring the Pregnancy Specific Glycoproteins (PSG) gene family. These proteins play a critical role in pregnancy, with participation in immunotolerance, angiogenesis, and invasion processes, which are also observed in carcinogenesis. The aim of this study was to determine the molecular alterations of PSG1 and its relationship with CC. Methods: PSG1 Copy Number Variation (CNV) was evaluated in 31 CC and eight normal cervical tissues by qPCR. PSG1 expression was correlated with HPV detection and IL-10 and TGF-β expression in CC samples. Finally, PSG1 protein expression was evaluated by immunofluorescence in CC cell lines, by immunohistochemistry in a tissue microarray, and by immunoblotting in the sera of women with normal cervix, pre-invasive lesions, and CC. Results: PSG1 showed a gain of 25.6% in CNV and gene expression in CC. There was a lack of PSG1 expression in normal cervical epithelium and positive immunostaining in 57% of CC tissues, while all CC cell lines expressed PSG1. Finally, PSG1 was immunodetected in 90% of pre-invasive lesions and in all CC serum samples, but not in healthy women. PSG1 expression correlates with the expression of IL-10 and TGF-β in CC tissues, but not with the presence of HPV. Conclusion: These data show evidence of the differential expression of PSG1 in CC that could explain its participation in tumor-biology and immunotolerance mechanisms. Further, its immunodetection could provide early detection of this cancer.
KW - Anti-inflammatory
KW - Cervical cancer
KW - Immunodetection
KW - PSG1
UR - http://www.scopus.com/inward/record.url?scp=85086510407&partnerID=8YFLogxK
U2 - 10.1016/j.arcmed.2020.05.025
DO - 10.1016/j.arcmed.2020.05.025
M3 - Artículo
C2 - 32546445
AN - SCOPUS:85086510407
SN - 0188-4409
VL - 51
SP - 504
EP - 514
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 6
ER -