TY - JOUR
T1 - Expression of functional interleukin-12 from mouse in transgenic tomato plants
AU - Gutiérrez-Ortega, Abel
AU - Sandoval-Montes, Claudia
AU - De Olivera-Flores, Teresa Jesús
AU - Santos-Argumedo, Leopoldo
AU - Gómez-Lim, Miguel Ángel
N1 - Funding Information:
We thankfully acknowledge the technical assistance of Luis Jorge Saucedo-Arias, M.Sc. Hector Romero-Ramirez and MVZ Ricardo Gaxiola-Centeno. This research was partially supported by a CONACyT Grant (#28772-N) to M.A.G.L. We are indebted to CONACYT for a PhD scholarship to A.G.O. (#128924). We thank Dr. C. Jiang for the kind gift of the mIL-12 fusion gene.
PY - 2005/12
Y1 - 2005/12
N2 - Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of a cytokine with immunomodulatory function, mouse interleukin-12 (IL-12), in transgenic tomato plants. Single-chain mouse IL-12 driven by the CaMV 35S promoter, accumulates to high levels in leaves and fruits (up to 7.3 and 3.4 μg per gram of fresh weight, respectively). Mouse IL-12 expressed in tomato displays biological activity in vitro, as determined by interferon-γ (IFN-γ) secretion by T cells. Possible uses of this plant-based cytokine involving mucosal delivery are discussed.
AB - Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of a cytokine with immunomodulatory function, mouse interleukin-12 (IL-12), in transgenic tomato plants. Single-chain mouse IL-12 driven by the CaMV 35S promoter, accumulates to high levels in leaves and fruits (up to 7.3 and 3.4 μg per gram of fresh weight, respectively). Mouse IL-12 expressed in tomato displays biological activity in vitro, as determined by interferon-γ (IFN-γ) secretion by T cells. Possible uses of this plant-based cytokine involving mucosal delivery are discussed.
KW - Interferon gamma
KW - Interleukin-12
KW - Recombinant proteins
KW - Tomato
KW - Transgenic plants
UR - http://www.scopus.com/inward/record.url?scp=28244442522&partnerID=8YFLogxK
U2 - 10.1007/s11248-005-1464-8
DO - 10.1007/s11248-005-1464-8
M3 - Artículo
SN - 0962-8819
VL - 14
SP - 877
EP - 885
JO - Transgenic Research
JF - Transgenic Research
IS - 6
ER -