TY - JOUR
T1 - Evaluation of the effect of heat damage on DNA extracted from the dental pulp of restored teeth
AU - Estrada, Lucía Angeles
AU - Martínez-Cuazitl, Adriana
AU - Mata-Miranda, Mónica Maribel
AU - González-Díaz, César Antonio
AU - Vázquez-Zapién, Gustavo Jesús
AU - Saldaña-Rivera, Elsa
AU - Sánchez-Monroy, Virginia
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/3
Y1 - 2019/3
N2 - We evaluate structural damage effects of heat on DNA obtained from the dental pulp of restored premolars. We studied three groups (A, B and C) each group comprised twenty premolars extracted from five patients. Three of the four premolars of each donator were restored with different materials: amalgam, glass ionomer and resin, and one unrestored premolar was used as control. The group A was not exposed to heat, while B and C groups were exposed to 100 °C and 300 °C, respectively. The DNA damage was evaluated as percentage of genotyping of 15 Short Tandem Repeats (STRs) and amelogenin and by Fourier-transform infrared spectroscopy (FTIR). The results showed 100% genotyping in all unheated premolars; however, the increase in heat decreased genotyping percentage due to DNA degradation. The amplifications from the premolars restored with glass ionomer and those unrestored were less affected, amplifying by approximately 80% at 300 °C. FTIR revealed that DNA structural damage occurred in the phosphate region, and changes in ribose were also shown; in addition, we detected presence of β- three-calcium-phosphate (β – TCP) due to heat treatment. Moreover, the phosphate region of DNA was a good indicator of DNA integrity related to the ratio of 1230/1085 cm −1 in the second derivative (asymmetric/symmetric PO2), which was major in premolars restored with glass ionomer heated at 100 °C, and this ratio is related to less DNA alterations and better genotyping; however this changes only were detected at 100 °C, suggesting that dental restoration with this material only protects dental pulp at temperatures below 300 °C.
AB - We evaluate structural damage effects of heat on DNA obtained from the dental pulp of restored premolars. We studied three groups (A, B and C) each group comprised twenty premolars extracted from five patients. Three of the four premolars of each donator were restored with different materials: amalgam, glass ionomer and resin, and one unrestored premolar was used as control. The group A was not exposed to heat, while B and C groups were exposed to 100 °C and 300 °C, respectively. The DNA damage was evaluated as percentage of genotyping of 15 Short Tandem Repeats (STRs) and amelogenin and by Fourier-transform infrared spectroscopy (FTIR). The results showed 100% genotyping in all unheated premolars; however, the increase in heat decreased genotyping percentage due to DNA degradation. The amplifications from the premolars restored with glass ionomer and those unrestored were less affected, amplifying by approximately 80% at 300 °C. FTIR revealed that DNA structural damage occurred in the phosphate region, and changes in ribose were also shown; in addition, we detected presence of β- three-calcium-phosphate (β – TCP) due to heat treatment. Moreover, the phosphate region of DNA was a good indicator of DNA integrity related to the ratio of 1230/1085 cm −1 in the second derivative (asymmetric/symmetric PO2), which was major in premolars restored with glass ionomer heated at 100 °C, and this ratio is related to less DNA alterations and better genotyping; however this changes only were detected at 100 °C, suggesting that dental restoration with this material only protects dental pulp at temperatures below 300 °C.
KW - DNA fingerprinting
KW - Dental pulp
KW - Dental restoration
KW - FTIR
KW - Glass ionomer
KW - STRs
UR - http://www.scopus.com/inward/record.url?scp=85060116815&partnerID=8YFLogxK
U2 - 10.1016/j.legalmed.2019.01.006
DO - 10.1016/j.legalmed.2019.01.006
M3 - Artículo
C2 - 30660659
SN - 1344-6223
VL - 37
SP - 54
EP - 59
JO - Legal Medicine
JF - Legal Medicine
ER -