Evaluation of the antidiabetic potential of extracts of urtica dioica, apium graveolens, and zingiber officinale in mice, zebrafish, and pancreatic β-cell

Rosa Martha Pérez Gutiérrez, Alethia Muñiz-Ramirez, Abraham Heriberto Garcia-Campoy, José María Mota Flores

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Medicinal plants are commonly used in the treatment of diabetes, particularly as they contain flavonoids and phenolic compounds. The present study aims to investigate the activities of a polyherbal formulation made from Urtica dioica, Apium graveolens, and Zingiber officinale (UAZ) against streptozotocin–nicotinamide ((STZ-NA)-induced type 2 diabetes in CD1 mice, glucose-induced type 2 diabetes (T2DM) in zebrafish, and high glucose-induced damage in RINm5F pancreatic β-cells. In fasting mice, plasma glucose, glycosylated hemoglobin (HbA1C), lipid hydroperoxides (LOOH), thiobarbituric acid reactive substances (TBARS), and lipid profiles were significantly increased, whereas insulin, enzymatic antioxidants, and carbohydrate metabolic enzymes were altered significantly in diabetic mice. Zebrafish had similar glucose levels, liver enzymes, and lipid profiles compared to mice. The study investigated the effects of the extract in enhancing cell viability, insulin secretion, and reducing lipid peroxidation and intracellular reactive oxygen species (ROS) levels in RINm5F cells damaged by high glucose. All the above biochemical parameters were enhanced in both mice and zebrafish treated; the combined extract UAZ normalized all the biochemical parameters. The medicinal plant extracts, used either separately or in combination, ameliorated the adverse effect of glucose on cell viability and functionality of beta-RINm5F cells.

Original languageEnglish
Article number1438
JournalPlants
Volume10
Issue number7
DOIs
StatePublished - Jul 2021
Externally publishedYes

Keywords

  • Apium graveolens
  • Diabetes
  • Mice
  • Pancreatic βRINm5F cell
  • Urtica dioica
  • Zebrafish
  • Zingiber officinale

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