TY - JOUR
T1 - Phytochemical, cytotoxic, and genotoxic evaluation of protein extract of Amaranthus hypochondriacus seeds
AU - Valadez-Vega, Carmen
AU - Lugo-Magaña, Olivia
AU - Morales-González, José A.
AU - Delgado-Olivares, Luis
AU - Izquierdo-Vega, Jeannett A.
AU - Sánchez-Gutiérrez, Manuel
AU - López-Contreras, Luilli
AU - Bautista, Mirandeli
AU - Velázquez-González, Claudia
N1 - Publisher Copyright:
© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.
PY - 2021
Y1 - 2021
N2 - Amaranth has been of interest for its nutritional quality and its multiple benefits; however, there is little research on its toxicological effect. This work studied antinutritional compounds, the cytotoxic and genotoxic effect in the Amaranthus protein extract (PEAh). The content of lectins, tannins, saponins, and trypsin inhibitors was determined. Biological studies were conducted to determine toxicity in male CD-1 mice by intraperitoneal administration. The genotoxic potential was determined by the micronucleus test. The bone marrow cytotoxicity was determined by polychromatic erythrocyte (PE) rate and the cytotoxic effect was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Our results showed considerably high concentrations of lectins and trypsin inhibitors, lower concentrations of tannins and saponins in PEAh. The PEAh was toxic to mice, presenting genotoxic and cytotoxic damage to bone marrow and hepatocytes.
AB - Amaranth has been of interest for its nutritional quality and its multiple benefits; however, there is little research on its toxicological effect. This work studied antinutritional compounds, the cytotoxic and genotoxic effect in the Amaranthus protein extract (PEAh). The content of lectins, tannins, saponins, and trypsin inhibitors was determined. Biological studies were conducted to determine toxicity in male CD-1 mice by intraperitoneal administration. The genotoxic potential was determined by the micronucleus test. The bone marrow cytotoxicity was determined by polychromatic erythrocyte (PE) rate and the cytotoxic effect was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Our results showed considerably high concentrations of lectins and trypsin inhibitors, lower concentrations of tannins and saponins in PEAh. The PEAh was toxic to mice, presenting genotoxic and cytotoxic damage to bone marrow and hepatocytes.
KW - Amaranthus hypochondriacus
KW - antinutritional
KW - cytotoxic
KW - genotoxic
KW - protein extract
UR - http://www.scopus.com/inward/record.url?scp=85114876829&partnerID=8YFLogxK
U2 - 10.1080/19476337.2021.1971771
DO - 10.1080/19476337.2021.1971771
M3 - Artículo
AN - SCOPUS:85114876829
SN - 1947-6337
VL - 19
SP - 701
EP - 709
JO - CYTA - Journal of Food
JF - CYTA - Journal of Food
IS - 1
ER -