TY - JOUR
T1 - Estrous cycle and gestational age-dependent expression of members of the interleukin-36 subfamily in a semi-allogeneic model of infected and non-infected murine pregnancy
AU - Murrieta-Coxca, José Martin
AU - Gómez-Chávez, Fernando
AU - Baeza-Martínez, Damariz Adriana
AU - Cancino-Diaz, Mario Eugenio
AU - Cancino-Diaz, Juan Carlos
AU - Pérez-Tapia, Sonia Mayra
AU - Reyes-Maldonado, Elba
AU - Rodríguez-Martínez, Sandra
N1 - Publisher Copyright:
© 2016 Murrieta-Coxca, Gómez-Chávez, Baeza-Martínez, Cancino-Diaz, Cancino-Diaz, Pérez-Tapia, Reyes-Maldonado and Rodríguez-Martínez.
PY - 2016/9/22
Y1 - 2016/9/22
N2 - The IL-36 subfamily is a recently described group of cytokines with pro-inflammatory behavior, comprising three agonists (α, β, and γ), its receptor (R), and one antagonist (Ra). The expression and function of IL-36 subfamily members in the estrous cycle in healthy and infected pregnancy has not been described. We evaluated mRNA and protein expression of IL-36 family members during the estrous cycle, implantation, fetal development, and post-labor periods in a model of allogenic pregnancy in mice. We also explored the ability of Listeria monocytogenes to modulate the expression of IL-36 subfamily members during pregnancy. Expression of IL-36 subfamily members showed different expression during the estrous cycle and pregnancy but was induced at estrous, 16.5 days post coitum (dpc), 18.5 dpc, and labor. IL-36 subfamily members showed a characteristic distribution in the glandular epithelium, perimetrium, myometrium, and stratum vasculare. Infection with L. monocytogenes during pregnancy induced strong production of IL-36 subfamily members, an observation that correlated with an increasing prevalence of fetal loss. In conclusion, IL-36 agonists showed specific patterns of mRNA and protein expression that might suggest functional specialization or specific target cells. Infection with L. monocytogenes during pregnancy induced strong production of IL-36 subfamily members.
AB - The IL-36 subfamily is a recently described group of cytokines with pro-inflammatory behavior, comprising three agonists (α, β, and γ), its receptor (R), and one antagonist (Ra). The expression and function of IL-36 subfamily members in the estrous cycle in healthy and infected pregnancy has not been described. We evaluated mRNA and protein expression of IL-36 family members during the estrous cycle, implantation, fetal development, and post-labor periods in a model of allogenic pregnancy in mice. We also explored the ability of Listeria monocytogenes to modulate the expression of IL-36 subfamily members during pregnancy. Expression of IL-36 subfamily members showed different expression during the estrous cycle and pregnancy but was induced at estrous, 16.5 days post coitum (dpc), 18.5 dpc, and labor. IL-36 subfamily members showed a characteristic distribution in the glandular epithelium, perimetrium, myometrium, and stratum vasculare. Infection with L. monocytogenes during pregnancy induced strong production of IL-36 subfamily members, an observation that correlated with an increasing prevalence of fetal loss. In conclusion, IL-36 agonists showed specific patterns of mRNA and protein expression that might suggest functional specialization or specific target cells. Infection with L. monocytogenes during pregnancy induced strong production of IL-36 subfamily members.
KW - Epithelium
KW - Estrous cycle
KW - IL-36
KW - Inflammation
KW - Listeria monocytogenes
KW - MRNA
KW - Pregnancy
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=85053361484&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2016.00376
DO - 10.3389/fimmu.2016.00376
M3 - Artículo
AN - SCOPUS:85053361484
SN - 1664-3224
VL - 7
JO - Frontiers in Immunology
JF - Frontiers in Immunology
IS - SEP
M1 - 376
ER -