TY - JOUR
T1 - Estrogenic, anti-estrogenic and cytotoxic effects elicited by water from the type localities of the endangered goodeid fish Girardinichthys viviparus
AU - Vega-López, Armando
AU - Ramón-Gallegos, Eva
AU - Galar-Martínez, Marcela
AU - Jiménez-Orozco, F. Alejandro
AU - García-Latorre, Ethel
AU - Domínguez-López, Maria Lilia
N1 - Funding Information:
We are grateful to SEMARNAT, Dirección General de Vida Silvestre (México) for authorizing the collection of parent specimens. Our thanks also to the staff of the Lago de Texcoco project, CNA, in particular MVZ Fernando Núñez for the support in the field. This study was financed by the Instituto Politécnico Nacional, CGPI code 20041154. Eva Ramón-Gallegos, Marcela Galar-Martínez, Ethel García-Latorre and Maria Lilia Domínguez-López are fellows of COFAA-IPN, EDI and SNI.
PY - 2007/4
Y1 - 2007/4
N2 - Girardinichthys viviparus is a Mexican endangered endemic fish species living only in Lake Texcoco (LTX), one of two extant type localities for this species. The other type locality is Lake Zumpango (LZ). LTX and LZ are fed by wastewater treated at secondary level that contains several endocrine disrupting chemicals. Our goal was to assess the estrogenic and anti-estrogenic effects elicited in G. viviparus by water from the two type localities and by these same matrices enriched with PCBs in order to understand potential damage due to increased xenobiotic levels. Estrogenic and anti-estrogenic effects were evaluated in vitro by E-screen assay in MCF-7 cells and cytotoxicity by MTT assay. PCBs were quantified in type localities. In vivo vitellogenin (VTG) induction was determined by a hybrid ELISA in adult laboratory-born fish exposed during 1, 2, 4, 8 and 16 days to LTX or LZ water in static exposure systems, and by the same matrices enriched with PCBs. We found PCBs only in LTX, but the water from both type localities elicit estrogenic and anti-estrogenic effects in vitro. Cytotoxicity was not observed in MCF-7 cells exposed to LTX or LZ water. VTG induction was higher with LTX water than with LZ water; also the response of males was greater than in females. In the PCB-enriched matrices, VTG induction in both sexes exposed to LTX water was reduced compared to un-enriched matrices. Thus, the sublethal increases in PCB levels may be hazardous to both sexes since they are linked probably to hepatotoxicity.
AB - Girardinichthys viviparus is a Mexican endangered endemic fish species living only in Lake Texcoco (LTX), one of two extant type localities for this species. The other type locality is Lake Zumpango (LZ). LTX and LZ are fed by wastewater treated at secondary level that contains several endocrine disrupting chemicals. Our goal was to assess the estrogenic and anti-estrogenic effects elicited in G. viviparus by water from the two type localities and by these same matrices enriched with PCBs in order to understand potential damage due to increased xenobiotic levels. Estrogenic and anti-estrogenic effects were evaluated in vitro by E-screen assay in MCF-7 cells and cytotoxicity by MTT assay. PCBs were quantified in type localities. In vivo vitellogenin (VTG) induction was determined by a hybrid ELISA in adult laboratory-born fish exposed during 1, 2, 4, 8 and 16 days to LTX or LZ water in static exposure systems, and by the same matrices enriched with PCBs. We found PCBs only in LTX, but the water from both type localities elicit estrogenic and anti-estrogenic effects in vitro. Cytotoxicity was not observed in MCF-7 cells exposed to LTX or LZ water. VTG induction was higher with LTX water than with LZ water; also the response of males was greater than in females. In the PCB-enriched matrices, VTG induction in both sexes exposed to LTX water was reduced compared to un-enriched matrices. Thus, the sublethal increases in PCB levels may be hazardous to both sexes since they are linked probably to hepatotoxicity.
KW - E-screen assay
KW - EDCs
KW - MCF-7
KW - PCBs
KW - Treated wastewater
KW - Vitellogenin
UR - http://www.scopus.com/inward/record.url?scp=33947646937&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2007.01.008
DO - 10.1016/j.cbpc.2007.01.008
M3 - Artículo
C2 - 17317326
SN - 1532-0456
VL - 145
SP - 394
EP - 403
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 3
ER -