Establishment of a hybrid SalI-HgiDII type II restriction-modification system

Ana I. Pélaez, Rosa M. Ribas-Aparicio, Ana Gómez, M. Rosario Rodicio

Research output: Contribution to conferencePaper

Abstract

In the SalI system, endonuclease activity can be only achieved in the presence of a functional modification gene. Thus, the DNA methyltransferase is involved in the control of restriction. By fusion of the restriction gene of the SalI system to the modification gene of the isospecific HgiDII system a hybrid type II restriction/modification system was created. Although in the hybrid situation the level of endonuclease activity was significantly lower than in the natural system, the HgiDII modification enzyme clearly supports SalI restriction. The mechanism by which the two isospecific methyltransferases control restriction is currently understudy.
Original languageAmerican English
Pages583-584
Number of pages524
StatePublished - 1 Apr 1998
EventBiological Chemistry -
Duration: 1 Apr 1998 → …

Conference

ConferenceBiological Chemistry
Period1/04/98 → …

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    Pélaez, A. I., Ribas-Aparicio, R. M., Gómez, A., & Rodicio, M. R. (1998). Establishment of a hybrid SalI-HgiDII type II restriction-modification system. 583-584. Paper presented at Biological Chemistry, .