TY - JOUR
T1 - Establishment and characterization of Prosopis laevigata (Humb. & Bonpl. ex Willd) M.C. Johnst. cell suspension culture
T2 - A biotechnology approach for mesquite gum production
AU - Trejo-Espino, J. L.
AU - Rodríguez-Monroy, M.
AU - Vernon-Carter, E. J.
AU - Cruz-Sosa, F.
N1 - Funding Information:
Acknowledgments This work was financed by grant SIP 20100401 from IPN. Trejo-Espino JL is indebted to Instituto Politécnico Nacional (IPN) and CONACYT for the doctoral fellowship awarded.
PY - 2011/9
Y1 - 2011/9
N2 - This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige-Skoog medium with two different plant growth regulators (PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5. 0 μM) and kinetin (KIN; 5. 0 μM). With this PGRs treatment, after four subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell suspension culture showed a specific cell growth rate (μ) of 0. 14 d-1 and doubling time (td) of 6. 6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture and the broth from cell suspension culture showed the presence of Arabinogalactan-proteins, and their polysaccharide fraction presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three samples exhibited similar emulsifying capacity and emulsification stability.
AB - This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige-Skoog medium with two different plant growth regulators (PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5. 0 μM) and kinetin (KIN; 5. 0 μM). With this PGRs treatment, after four subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell suspension culture showed a specific cell growth rate (μ) of 0. 14 d-1 and doubling time (td) of 6. 6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture and the broth from cell suspension culture showed the presence of Arabinogalactan-proteins, and their polysaccharide fraction presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three samples exhibited similar emulsifying capacity and emulsification stability.
KW - Arabinogalactan-proteins
KW - Emulsifying capacity
KW - Mesquite
KW - Plant polysaccharides
UR - http://www.scopus.com/inward/record.url?scp=80051664215&partnerID=8YFLogxK
U2 - 10.1007/s11738-010-0705-5
DO - 10.1007/s11738-010-0705-5
M3 - Artículo
SN - 0137-5881
VL - 33
SP - 1687
EP - 1695
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 5
ER -