TY - JOUR
T1 - ESKAPE bacteria characterization reveals the presence of Acinetobacter baumannii and Pseudomonas aeruginosa outbreaks in COVID-19/VAP patients
AU - Loyola-Cruz, Miguel Ángel
AU - Durán-Manuel, Emilio Mariano
AU - Cruz-Cruz, Clemente
AU - Márquez-Valdelamar, Laura Margarita
AU - Bravata-Alcántara, Juan Carlos
AU - Cortés-Ortíz, Iliana Alejandra
AU - Cureño-Díaz, Mónica Alethia
AU - Ibáñez-Cervantes, Gabriela
AU - Fernández-Sánchez, Verónica
AU - Castro-Escarpulli, Graciela
AU - Bello-López, Juan Manuel
N1 - Publisher Copyright:
© 2022 Association for Professionals in Infection Control and Epidemiology, Inc.
PY - 2023/7
Y1 - 2023/7
N2 - Introduction: A decrease of detection of outbreaks by multidrug-resistant bacteria in critical areas has been reduced due to COVID-19 pandemic. Therefore, molecular epidemiological surveillance should be a primary tool to reveal associations not evident by classical epidemiology. The aim of this work was to demonstrate the presence of hidden outbreaks in the first wave of the COVID-19 pandemic and to associate their possible origin. Methods: A population of 96 COVID-19 patients was included in the study (April to June 2020) from Hospital Juárez de México. Genetic identification and antimicrobial susceptibility testing of VAP causative agents isolated from COVID-19 patients was performed. Resistance phenotypes were confirmed by PCR. Clonal association of isolates was performed by analysis of intergenic regions obtained. Finally, the association of clonal cases of VAP patients was performed by timelines. Results: ESKAPE and non-ESKAPE bacteria were identified as causative agents of VAP. ESKAPE bacteria were classified as MDR and XDR. Only A. baumannii and P. aeruginosa were identified as clonally distributed in 13 COVID-19/VAP patients. Time analysis showed that cross-transmission existed between patients and care areas. Conclusions: Acinetobacter baumannii and Pseudomonas aeruginosa were involved in outbreaks non-detected in COVID-19/VAP patients in the first wave of COVID-19 pandemic.
AB - Introduction: A decrease of detection of outbreaks by multidrug-resistant bacteria in critical areas has been reduced due to COVID-19 pandemic. Therefore, molecular epidemiological surveillance should be a primary tool to reveal associations not evident by classical epidemiology. The aim of this work was to demonstrate the presence of hidden outbreaks in the first wave of the COVID-19 pandemic and to associate their possible origin. Methods: A population of 96 COVID-19 patients was included in the study (April to June 2020) from Hospital Juárez de México. Genetic identification and antimicrobial susceptibility testing of VAP causative agents isolated from COVID-19 patients was performed. Resistance phenotypes were confirmed by PCR. Clonal association of isolates was performed by analysis of intergenic regions obtained. Finally, the association of clonal cases of VAP patients was performed by timelines. Results: ESKAPE and non-ESKAPE bacteria were identified as causative agents of VAP. ESKAPE bacteria were classified as MDR and XDR. Only A. baumannii and P. aeruginosa were identified as clonally distributed in 13 COVID-19/VAP patients. Time analysis showed that cross-transmission existed between patients and care areas. Conclusions: Acinetobacter baumannii and Pseudomonas aeruginosa were involved in outbreaks non-detected in COVID-19/VAP patients in the first wave of COVID-19 pandemic.
KW - Bacteria ESKAPE
KW - COVID-19
KW - Clonal dispersion
KW - ERIC-PCR
KW - Ventilator associated pneumonia
UR - http://www.scopus.com/inward/record.url?scp=85138219853&partnerID=8YFLogxK
U2 - 10.1016/j.ajic.2022.08.012
DO - 10.1016/j.ajic.2022.08.012
M3 - Artículo
C2 - 36002081
AN - SCOPUS:85138219853
SN - 0196-6553
VL - 51
SP - 729
EP - 737
JO - American Journal of Infection Control
JF - American Journal of Infection Control
IS - 7
ER -