TY - JOUR
T1 - Ergosterol peroxide isolated from oyster medicinal mushroom, pleurotus ostreatus (Agaricomycetes), potentially induces radiosensitivity in cervical cancer
AU - Meza-Menchaca, Thuluz
AU - Poblete-Naredo, Irais
AU - Albores-Medina, Arnulfo
AU - Pedraza-Chaverri, José
AU - Quiroz-Figueroa, Francisco R.
AU - Cruz-Gregorio, Alfredo
AU - Zepeda, Rossana C.
AU - Melgar-Lalanne, Guiomar
AU - Lagunes, Irene
AU - Trigos, Ángel
N1 - Publisher Copyright:
© 2020 by Begell House, Inc. www.begellhouse.com.
PY - 2020
Y1 - 2020
N2 - Every year, more than 500,000 new cases of cervical cancer are reported, making it the fourth lead-ing cause of cancer globally. Although human papillomavirus (HPV) vaccines show promise as a protective measure, HPV-related cancers remain a public health problem since the vaccines, which are only specific to certain viral types, are unavailable for mass distribution. Furthermore, the effects of toxicity following ionizing radiation therapy have reori-ented views toward the search for radiosensitizers that can reduce toxicity as a consequence of decreased radiation doses. Here, we isolated ergosterol peroxide (EP) from Pleurotus ostreatus and purified it to test its potential effects in vitro. We thus observed that a gradual increase in EP dose correlates with a loss of viability in HeLa and CaSki cervical cell lines. Dose/response curves were constructed using cervical cancer cell lines, as well as normal human peripheral blood mononuclear cells. The selectivity of EP in human lymphocytes and cervical cancer cell lines was tested, and no toxicity was found in normal cells. A combination of treatments revealed a radiosensitizer effect in HeLa cells, when measuring the exposure to EP followed by irradiation with137Cs. Our findings suggest that EP may be effective as a radiosensitizer in treating cervical cancer.
AB - Every year, more than 500,000 new cases of cervical cancer are reported, making it the fourth lead-ing cause of cancer globally. Although human papillomavirus (HPV) vaccines show promise as a protective measure, HPV-related cancers remain a public health problem since the vaccines, which are only specific to certain viral types, are unavailable for mass distribution. Furthermore, the effects of toxicity following ionizing radiation therapy have reori-ented views toward the search for radiosensitizers that can reduce toxicity as a consequence of decreased radiation doses. Here, we isolated ergosterol peroxide (EP) from Pleurotus ostreatus and purified it to test its potential effects in vitro. We thus observed that a gradual increase in EP dose correlates with a loss of viability in HeLa and CaSki cervical cell lines. Dose/response curves were constructed using cervical cancer cell lines, as well as normal human peripheral blood mononuclear cells. The selectivity of EP in human lymphocytes and cervical cancer cell lines was tested, and no toxicity was found in normal cells. A combination of treatments revealed a radiosensitizer effect in HeLa cells, when measuring the exposure to EP followed by irradiation with137Cs. Our findings suggest that EP may be effective as a radiosensitizer in treating cervical cancer.
KW - Cervical cancer
KW - Endoperoxide
KW - Ergosterol peroxide
KW - HPV
KW - Medicinal mushrooms
KW - Pleurotus ostreatus
KW - Radiosensitivity
UR - http://www.scopus.com/inward/record.url?scp=85096862526&partnerID=8YFLogxK
U2 - 10.1615/IntJMedMushrooms.2020036673
DO - 10.1615/IntJMedMushrooms.2020036673
M3 - Artículo
C2 - 33426842
AN - SCOPUS:85096862526
SN - 1521-9437
VL - 22
SP - 1109
EP - 1119
JO - International Journal of Medicinal Mushrooms
JF - International Journal of Medicinal Mushrooms
IS - 11
ER -