TY - JOUR
T1 - Epigenome-Wide Analysis Reveals DNA Methylation Alteration in ZFP57 and Its Target RASGFR2 in a Mexican Population Cohort with Autism
AU - Aspra, Queletzu
AU - Cabrera-Mendoza, Brenda
AU - Morales-Marín, Mirna Edith
AU - Márquez, Carla
AU - Chicalote, Carlos
AU - Ballesteros, Ana
AU - Aguilar, Miriam
AU - Castro, Xochitl
AU - Gómez-Cotero, Amalia
AU - Balboa-Verduzco, Ana María
AU - Albores-Gallo, Lilia
AU - Nafate-López, Omar
AU - Marcín-Salazar, Carlos Alfonso
AU - Sánchez, Patricia
AU - Lanzagorta-Piñol, Nuria
AU - López-Armenta, Fernando Omar
AU - Nicolini, Humberto
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/4
Y1 - 2022/4
N2 - Autism Spectrum Disorders (ASD) comprise a group of heterogeneous and complex neu-rodevelopmental disorders. Genetic and environmental factors contribute to ASD etiology. DNA methylation is particularly relevant for ASD due to its mediating role in the complex interaction between genotype and environment and has been implicated in ASD pathophysiology. The lack of diversity in DNA methylation studies in ASD individuals is remarkable. Since genetic and environmental factors are likely to vary across populations, the study of underrepresented populations is necessary to understand the molecular alterations involved in ASD and the risk factors underly-ing these changes. This study explored genome-wide differences in DNA methylation patterns in buccal epithelium cells between Mexican ASD patients (n = 27) and age-matched typically developing (TD: n = 15) children. DNA methylation profiles were evaluated with the Illumina 450k array. We evaluated the interaction between sex and ASD and found a differentially methylated region (DMR) over the 5′UTR region of ZFP57 and one of its targets, RASGRF2. These results match previous findings in brain tissue, which may indicate that ZFP57 could be used as a proxy for DNA methylation in different tissues. This is the first study performed in a Mexican, and subsequently, Latin American, population that evaluates DNA methylation in ASD patients.
AB - Autism Spectrum Disorders (ASD) comprise a group of heterogeneous and complex neu-rodevelopmental disorders. Genetic and environmental factors contribute to ASD etiology. DNA methylation is particularly relevant for ASD due to its mediating role in the complex interaction between genotype and environment and has been implicated in ASD pathophysiology. The lack of diversity in DNA methylation studies in ASD individuals is remarkable. Since genetic and environmental factors are likely to vary across populations, the study of underrepresented populations is necessary to understand the molecular alterations involved in ASD and the risk factors underly-ing these changes. This study explored genome-wide differences in DNA methylation patterns in buccal epithelium cells between Mexican ASD patients (n = 27) and age-matched typically developing (TD: n = 15) children. DNA methylation profiles were evaluated with the Illumina 450k array. We evaluated the interaction between sex and ASD and found a differentially methylated region (DMR) over the 5′UTR region of ZFP57 and one of its targets, RASGRF2. These results match previous findings in brain tissue, which may indicate that ZFP57 could be used as a proxy for DNA methylation in different tissues. This is the first study performed in a Mexican, and subsequently, Latin American, population that evaluates DNA methylation in ASD patients.
KW - Autism
KW - DNA methylation
KW - Mexican
KW - children
KW - imprinting
UR - http://www.scopus.com/inward/record.url?scp=85128012205&partnerID=8YFLogxK
U2 - 10.3390/children9040462
DO - 10.3390/children9040462
M3 - Artículo
C2 - 35455506
AN - SCOPUS:85128012205
SN - 2227-9067
VL - 9
JO - Children
JF - Children
IS - 4
M1 - 462
ER -