TY - JOUR
T1 - Entamoeba histolytica
T2 - DNA carrier vesicles in nuclei and kinetoplast-like organelles (EhkOs)
AU - Solís, F.
AU - Orozco, E.
AU - Córdova, L.
AU - Rivera, B.
AU - Luna-Arias, J.
AU - Gómez-Conde, E.
AU - Rodríguez, M.
N1 - Funding Information:
Acknowledgements The authors are deeply grateful for the excellent technical assistance of Mr. Alfredo Padilla-Barberi during the photographic work. The authors als o thank to the Unit of Electron Microscopy at CINVESTAV-IPN. This work was also supported by a Howard Hughes Medical Ins titute Grant awarded to Dr. E. Orozco, and by CONACyT (Mexico).
PY - 2002
Y1 - 2002
N2 - Entamoeba histolytica, the protozoan responsible for human amoebiasis, has a complex genome, whose linear chromosomes and DNA circles have so far eluded detailed analysis. We report the detection by transmission electron microscopy of nuclear vesicles (0.05-0.3 μm in diameter) carrying DNA in E. histolytica trophozoites. In late anaphase many of these nuclear vesicles were found to be organized in structures of ∼2.5×1 μm, in association with chromosomes and microtubules. In glutaraldehyde-fixed and detergent-treated trophozoites, nuclear vesicles displayed a non-membranous envelope. Binding of phosphotungstate stain and recognition by serum from patients with systemic lupus erythematosus indicated that these vesicles contain DNA. Similar DNA carrier vesicles were found in the cytoplasm and in the E. histolytica kinetoplast-like organelle (EhkO). By Feulgen staining, we detected DNA carrier vesicles entering or leaving the nuclei, suggesting a structural relationship between the nuclear vesicles and the vesicles present in the EhkOs.
AB - Entamoeba histolytica, the protozoan responsible for human amoebiasis, has a complex genome, whose linear chromosomes and DNA circles have so far eluded detailed analysis. We report the detection by transmission electron microscopy of nuclear vesicles (0.05-0.3 μm in diameter) carrying DNA in E. histolytica trophozoites. In late anaphase many of these nuclear vesicles were found to be organized in structures of ∼2.5×1 μm, in association with chromosomes and microtubules. In glutaraldehyde-fixed and detergent-treated trophozoites, nuclear vesicles displayed a non-membranous envelope. Binding of phosphotungstate stain and recognition by serum from patients with systemic lupus erythematosus indicated that these vesicles contain DNA. Similar DNA carrier vesicles were found in the cytoplasm and in the E. histolytica kinetoplast-like organelle (EhkO). By Feulgen staining, we detected DNA carrier vesicles entering or leaving the nuclei, suggesting a structural relationship between the nuclear vesicles and the vesicles present in the EhkOs.
KW - Cytoplasmic DNA
KW - DNA-carrying vesicles
KW - Entamoeba histolytica
KW - Kinetoplast-like organelles
KW - Nuclear division
UR - http://www.scopus.com/inward/record.url?scp=0036325480&partnerID=8YFLogxK
U2 - 10.1007/s00438-002-0693-2
DO - 10.1007/s00438-002-0693-2
M3 - Artículo
SN - 1617-4615
VL - 267
SP - 622
EP - 628
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 5
ER -