TY - JOUR
T1 - Entamoeba histolytica
T2 - Comparative genomics of the pre-mRNA 3′ end processing machinery
AU - López-Camarillo, César
AU - Orozco, Esther
AU - Marchat, Laurence A.
N1 - Funding Information:
This work was supported by CGPI-IPN (20040056) and CONACyT-México (39888-M) grants. Preliminary gene sequences were obtained from TIGR and Sanger E. histolytica genome databases. The TIGR and Sanger sequencing efforts are supported by awards from Institute of Allergy and Infectious Diseases, National Institutes of Health, USA, and from The Wellcome Trust, UK.
PY - 2005/7
Y1 - 2005/7
N2 - We report here the pre-mRNA 3′ end processing machinery in Entamoeba histolytica. Comparative analysis of the putative sequences participating in the pre-mRNA 3′ end processing of E. histolytica genes shows similitude and differences to those described for yeast and human transcripts. By a genomic survey, we identified 16 putative genes encoding for cleavage/polyadenylation factors in this parasite. E. histolytica pre-mRNA 3′ end processing machinery does not seem to contain homologous genes coding for human Symplekin, CFIm59, and CFIm68 proteins, neither sequences related to yeast Pta1p and Hrp1p. Protein sequence comparisons among E. histolytica, yeast, and human showed little variation in their functional domains through evolutive scale. E. histolytica pre-mRNA 3′ end processing machinery appears to be in an intermediate evolutionary position between mammals and yeast. From these analyses, we propose a hypothetical working model for the pre-mRNA 3′ end processing machinery in E. histolytica.
AB - We report here the pre-mRNA 3′ end processing machinery in Entamoeba histolytica. Comparative analysis of the putative sequences participating in the pre-mRNA 3′ end processing of E. histolytica genes shows similitude and differences to those described for yeast and human transcripts. By a genomic survey, we identified 16 putative genes encoding for cleavage/polyadenylation factors in this parasite. E. histolytica pre-mRNA 3′ end processing machinery does not seem to contain homologous genes coding for human Symplekin, CFIm59, and CFIm68 proteins, neither sequences related to yeast Pta1p and Hrp1p. Protein sequence comparisons among E. histolytica, yeast, and human showed little variation in their functional domains through evolutive scale. E. histolytica pre-mRNA 3′ end processing machinery appears to be in an intermediate evolutionary position between mammals and yeast. From these analyses, we propose a hypothetical working model for the pre-mRNA 3′ end processing machinery in E. histolytica.
UR - http://www.scopus.com/inward/record.url?scp=20444442702&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2005.02.024
DO - 10.1016/j.exppara.2005.02.024
M3 - Artículo
C2 - 15955310
AN - SCOPUS:20444442702
SN - 0014-4894
VL - 110
SP - 184
EP - 190
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 3 SPEC. ISS.
ER -