TY - JOUR
T1 - Engineering considerations to produce bioactive compounds from plant cell suspension culture in bioreactors
AU - Motolinía‐alcántara, Elizabeth Alejandra
AU - Castillo‐araiza, Carlos Omar
AU - Rodríguez‐monroy, Mario
AU - Román‐guerrero, Angélica
AU - Cruz‐sosa, Francisco
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - The large‐scale production of plant‐derived secondary metabolites (PDSM) in bioreactors to meet the increasing demand for bioactive compounds for the treatment and prevention of degenerative diseases is nowadays considered an engineering challenge due to the large number of operational factors that need to be considered during their design and scale‐up. The plant cell suspension culture (CSC) has presented numerous benefits over other technologies, such as the conventional whole‐plant extraction, not only for avoiding the overexploitation of plant species, but also for achieving better yields and having excellent scaling‐up attributes. The selection of the bioreactor configuration depends on intrinsic cell culture properties and engineering considerations related to the effect of operating conditions on thermodynamics, kinetics, and transport phenomena, which together are essential for accomplishing the largescale production of PDSM. To this end, this review, firstly, provides a comprehensive appraisement of PDSM, essentially those with demonstrated importance and utilization in pharmaceutical industries. Then, special attention is given to PDSM obtained out of CSC. Finally, engineering aspects related to the bioreactor configuration for CSC stating the effect of the operating conditions on kinetics and transport phenomena and, hence, on the cell viability and production of PDSM are presented accordingly. The engineering analysis of the reviewed bioreactor configurations for CSC will pave the way for future research focused on their scaling up, to produce high value‐added PDSM.
AB - The large‐scale production of plant‐derived secondary metabolites (PDSM) in bioreactors to meet the increasing demand for bioactive compounds for the treatment and prevention of degenerative diseases is nowadays considered an engineering challenge due to the large number of operational factors that need to be considered during their design and scale‐up. The plant cell suspension culture (CSC) has presented numerous benefits over other technologies, such as the conventional whole‐plant extraction, not only for avoiding the overexploitation of plant species, but also for achieving better yields and having excellent scaling‐up attributes. The selection of the bioreactor configuration depends on intrinsic cell culture properties and engineering considerations related to the effect of operating conditions on thermodynamics, kinetics, and transport phenomena, which together are essential for accomplishing the largescale production of PDSM. To this end, this review, firstly, provides a comprehensive appraisement of PDSM, essentially those with demonstrated importance and utilization in pharmaceutical industries. Then, special attention is given to PDSM obtained out of CSC. Finally, engineering aspects related to the bioreactor configuration for CSC stating the effect of the operating conditions on kinetics and transport phenomena and, hence, on the cell viability and production of PDSM are presented accordingly. The engineering analysis of the reviewed bioreactor configurations for CSC will pave the way for future research focused on their scaling up, to produce high value‐added PDSM.
KW - Bioactive compounds
KW - Bioreactor engineering
KW - Cell suspension culture (CSC)
KW - Medicinal plant
KW - Plant‐derived secondary metabolites (PDSM)
UR - http://www.scopus.com/inward/record.url?scp=85121041615&partnerID=8YFLogxK
U2 - 10.3390/plants10122762
DO - 10.3390/plants10122762
M3 - Artículo de revisión
C2 - 34961231
AN - SCOPUS:85121041615
SN - 2223-7747
VL - 10
JO - Plants
JF - Plants
IS - 12
M1 - 2762
ER -