TY - JOUR
T1 - Emetine produce Entamoeba histolytica death by inducing a programmed cell death
AU - Villalba-Magdaleno, J. D.
AU - Rojas, R.
AU - Gómez, C.
AU - Shibayama, M.
AU - Carrero, Julio C.
AU - Pérez Ishiwara, D. Guillermo
PY - 2007
Y1 - 2007
N2 - Amoebiasis is a major public health problem in tropical and subtropical countries. Although a number of antiamoebic agents are used for its treatment and the molecular targets of these drugs have been identified, the biochemical and physiological mechanisms that produce trophozoite death are little known. The present study dates the in vitro induction of programmed cell death (PCD) in E. histolytica by emetine. Morphologically, the emetine incubation reduces cytoplasmic volume, produce nuclear condensation and DNA fragmentation, maintaining the plasma membrane integrity; biochemically, the morphological changes are in concordance with the overproduction of reactive oxygen species inside trophozoites. These results provide the first insight related the cellular mechanisms of emetine action.
AB - Amoebiasis is a major public health problem in tropical and subtropical countries. Although a number of antiamoebic agents are used for its treatment and the molecular targets of these drugs have been identified, the biochemical and physiological mechanisms that produce trophozoite death are little known. The present study dates the in vitro induction of programmed cell death (PCD) in E. histolytica by emetine. Morphologically, the emetine incubation reduces cytoplasmic volume, produce nuclear condensation and DNA fragmentation, maintaining the plasma membrane integrity; biochemically, the morphological changes are in concordance with the overproduction of reactive oxygen species inside trophozoites. These results provide the first insight related the cellular mechanisms of emetine action.
KW - Apoptosis
KW - Emetine
KW - Entamoeba histolytica
KW - Programmed cell death
UR - http://www.scopus.com/inward/record.url?scp=43949111297&partnerID=8YFLogxK
U2 - 10.3844/ajidsp.2007.110.114
DO - 10.3844/ajidsp.2007.110.114
M3 - Artículo
SN - 1553-6203
VL - 3
SP - 110
EP - 114
JO - American Journal of Infectious Diseases
JF - American Journal of Infectious Diseases
IS - 2
ER -